首页|A mutation in the promoter of the yellow stripe-like transporter gene in cucumber results in a yellow cotyledon phenotype

A mutation in the promoter of the yellow stripe-like transporter gene in cucumber results in a yellow cotyledon phenotype

扫码查看
原文链接
  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
Leaf color mutants in higher plants are considered to be ideal materials for studying the chlorophyll biosynthesis,photosynthesis mechanism and chloroplast development.Herein,we identified a spontaneous mutant,yc412,in cultivated cucumber that exhibited yellow cotyledons.The yellow-lethal mutant was diagnosed with an abnormal chloroplast ultrastructure,and reduced photosynthetic capacity and pigment content.Through bulked segregant analysis-based whole-genome sequencing and fine genetic mapping,we narrowed the yellow cotyledons(yc)locus to a 96.8 kb interval on chromosome 3.By resequencing and molecular cloning,we showed that Csyc is a potential candidate gene,which encodes a yellow stripe-like(YSL)transporter.The T to C mutation in the promoter region of Csyc caused the yellow cotyledon phenotype in yc412.Compared to YZU027A(WT),the expression of Csyc was significantly downregulated in the cotyledons of yc412.Silencing of Csyc in cucumber via virus-induced gene silencing resulted in chlorotic leaves,mainly by suppressing the chlorophyll content.Furthermore,a comparative transcriptome analysis revealed that chloroplast-related genes and chlorophyll biosynthesis genes were significantly downregulated in yc412 cotyledons.Our results provide new insights into the molecular function of the YSL transporter in plant chloroplast development and chlorophyll synthesis.

cucumberyellow-lethal cotyledonschloroplast developmentyellow stripe-like transporter

Jiawei Pan、Jia Song、Rahat Sharif、Xuewen Xu、Shutong Li、Xuehao Chen

展开 >

School of Horticulture and Landscape Architecture,Yangzhou University,Yangzhou 225009,China

Institute of Vegetables,Tianjin Academy of Agricultural Sciences,Tianjin 300384,China

the"JBGS"Project of Seed Industry Revitalization in Jiangsu Province,ChinaJiangsu Agricultural Innovation of New Cultivars,ChinaOpen Project Program of Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement,China

JBGS[2021]018PZCZ201720K2020030

2024

10.1016/j.jia.2023.11.024

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
年,卷(期)：2024.23(3)
  • 67