首页|Alternative splicing of the PECTINESTERASE gene encoding a cell wall-degrading enzyme affects postharvest softening in grape

Alternative splicing of the PECTINESTERASE gene encoding a cell wall-degrading enzyme affects postharvest softening in grape

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  • 国家科技期刊平台
  • NETL
  • NSTL
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The firmness of table grape berries is a crucial quality parameter.Despite extensive research on postharvest fruit softening,its precise molecular mechanisms remain elusive.To enhance our comprehension of the underlying molecular factors,we initially identified differentially expressed genes(DEGs)by comparing the transcriptomes of folic acid(FA)-treated and water-treated(CK)berries at different time points.We then analyzed the sequences to detect alternatively spliced(AS)genes associated with postharvest softening.A total of 2,559 DEGs were identified and categorized into four subclusters based on their expression patterns,with subcluster-4 genes exhibiting higher expression in the CK group compared with the FA treatment group.There were 1,045 AS-associated genes specific to FA-treated berries and 1,042 in the CK-treated berries,respectively.Gene Ontology(GO)annotation indicated that the AS-associated genes in CK-treated berries were predominantly enriched in cell wall metabolic processes,particularly cell wall degradation processes.Through a comparison between treatment-associated AS genes and subcluster-4 DEGs,we identified eight genes,including Pectinesterase 2(VvPE2,Vitvi15g00704),which encodes a cell wall-degrading enzyme and was predicted to undergo an A3SS event.The reverse transcription polymerase chain reaction further confirmed the presence of a truncated transcript variant of VvPE2 in the FA-treated berries.Our study provides a comprehensive analysis of AS events in postharvest grape berries using transcriptome sequencing and underscores the pivotal role of VvPE2 during the postharvest storage of grape berries.

grapepostharvest softeningfolic acidalternative splicingPectinesterase 2alternative 3´ splice site(A3SS)

Hainan Liu、Maosong Pei、Charles Ampomah-Dwamena、Yaxin Shang、Yihe Yu、Tonglu Wei、Qiaofang Shi、Dalong Guo

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College of Horticulture and Plant Protection,Henan University of Science and Technology,Luoyang 471000,China

Henan Engineering Technology Research Center of Quality Regulation and Control of Horticultural Plants,Luoyang 471000,China

The New Zealand Institute for Plant&Food Research Limited(PFR),Auckland 1010,New Zealand

National Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaProgram for Innovative Research Team(in Science and Technology)in University of Henan Province,ChinaNatural Science Foundation of Henan,ChinaProgram for Science&Technology Innovation Talents in Universities of Henan Province,ChinaPhD Research Startup Foundation of Henan University of Science and Technology,ChinaPhD Research Startup Foundation of Henan University of Science and Technology,China

322025603230247021IRTSTHN02123230042111221HASTIT0351348006813480067

2024

10.1016/j.jia.2023.11.023

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
年,卷(期)：2024.23(3)
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