Alternative splicing of the PECTINESTERASE gene encoding a cell wall-degrading enzyme affects postharvest softening in grape

Hainan Liu ¹Maosong Pei ¹Charles Ampomah-Dwamena ²Yaxin Shang ¹Yihe Yu ¹Tonglu Wei ¹Qiaofang Shi ¹Dalong Guo¹

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作者信息

1. College of Horticulture and Plant Protection,Henan University of Science and Technology,Luoyang 471000,China;Henan Engineering Technology Research Center of Quality Regulation and Control of Horticultural Plants,Luoyang 471000,China
2. The New Zealand Institute for Plant&Food Research Limited(PFR),Auckland 1010,New Zealand
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Abstract

The firmness of table grape berries is a crucial quality parameter.Despite extensive research on postharvest fruit softening,its precise molecular mechanisms remain elusive.To enhance our comprehension of the underlying molecular factors,we initially identified differentially expressed genes(DEGs)by comparing the transcriptomes of folic acid(FA)-treated and water-treated(CK)berries at different time points.We then analyzed the sequences to detect alternatively spliced(AS)genes associated with postharvest softening.A total of 2,559 DEGs were identified and categorized into four subclusters based on their expression patterns,with subcluster-4 genes exhibiting higher expression in the CK group compared with the FA treatment group.There were 1,045 AS-associated genes specific to FA-treated berries and 1,042 in the CK-treated berries,respectively.Gene Ontology(GO)annotation indicated that the AS-associated genes in CK-treated berries were predominantly enriched in cell wall metabolic processes,particularly cell wall degradation processes.Through a comparison between treatment-associated AS genes and subcluster-4 DEGs,we identified eight genes,including Pectinesterase 2(VvPE2,Vitvi15g00704),which encodes a cell wall-degrading enzyme and was predicted to undergo an A3SS event.The reverse transcription polymerase chain reaction further confirmed the presence of a truncated transcript variant of VvPE2 in the FA-treated berries.Our study provides a comprehensive analysis of AS events in postharvest grape berries using transcriptome sequencing and underscores the pivotal role of VvPE2 during the postharvest storage of grape berries.

Key words

grape/postharvest softening/folic acid/alternative splicing/Pectinesterase 2/alternative 3´ splice site(A3SS)

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基金项目

National Natural Science Foundation of China(32202560)

National Natural Science Foundation of China(32302470)

Program for Innovative Research Team(in Science and Technology)in University of Henan Province,China(21IRTSTHN021)

Natural Science Foundation of Henan,China(232300421112)

Program for Science&Technology Innovation Talents in Universities of Henan Province,China(21HASTIT035)

PhD Research Startup Foundation of Henan University of Science and Technology,China(13480068)

PhD Research Startup Foundation of Henan University of Science and Technology,China(13480067)

出版年

2024

农业科学学报(英文)

中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD

影响因子：0.576

ISSN：2095-3119

参考文献量49

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