首页|Silencing transformer and transformer-2 in Zeugodacus cucurbitae causes defective sex determination with inviability of most pseudomales

Silencing transformer and transformer-2 in Zeugodacus cucurbitae causes defective sex determination with inviability of most pseudomales

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transformer is a switch gene for sex determination in many insects,which cooperates with transformer-2 that is expressed in both sexes to regulate female differentiation,particularly in dipterans.Zeugodacus cucurbitae(Coquillett)is a very destructive pest worldwide,however,its sex determination pathway remains largely uncharacterized.Here,we show that the female sex ratio is sharply reduced with knockdown of either transformer or transformer-2 by RNA interference in early embryos of Z.cucurbitae.Most of the males grown from the embryos with transient transformer and transformer-2 suppression mated with wild-type females and produced mixed sex progeny,with one exception that produced only female progeny,and all of the few remaining males failed to mate with wild-type females and produced no progeny.The exceptional male and those males with mating failure were XX pseudomales as determined by the detection of Y chromosome-linked Maleness-on-the-Y,indicating that most XX pseudomales are not viable.The phenotypes of transformer and transformer-2 suggest that they play a key role in regulating sex determination and are required for female sexual development of Z.cucurbitae.Our results will be beneficial to the understanding of sex determination in Z.cucurbitae and can facilitate the development of genetic sexing strains for its biological control.

Zeugodacus cucurbitaetransformertransformer-2sex determinationRNA interferencebiological control

Qin Ma、Zizhen Fan、Ping Wang、Siya Ma、Jian Wen、Fengqin Cao、Xianwu Lin、Rihui Yan

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Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests of Ministry of Education,School of Plant Protection,Hainan University,Haikou 570228,China

School of Life Sciences,Hainan University,Haikou 570228,China

Hainan Yazhou Bay Seed Lab,Sanya 572000,China

Hainan Provincial Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Natural Science Foundation of ChinaNational Key R&D Program of China

321CXTD435318605233166033931702059322606652022YFC2601400

2024

10.1016/j.jia.2023.06.019

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
年,卷(期)：2024.23(3)
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