首页|Fine mapping and cloning of the sterility gene Bra2Ms in non-heading Chinese cabbage(Brassica rapa ssp.chinensis)

Fine mapping and cloning of the sterility gene Bra2Ms in non-heading Chinese cabbage(Brassica rapa ssp.chinensis)

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
The application of a male-sterile line is an ideal approach for hybrid seed production in non-heading Chinese cabbage(Brassica rapa ssp.chinensis).However,the molecular mechanisms underlying male sterility in B.rapa are still largely unclear.We previously obtained the natural male sterile line WS24-3 of non-heading Chinese cabbage and located the male sterile locus,Bra2Ms,on the A2 chromosome.Cytological observations revealed that the male sterility of WS24-3 resulted from disruption of the meiosis process during pollen formation.Fine mapping of Bra2Ms delimited the locus within a physical distance of about 129 kb on the A2 chromosome of B.rapa.The Bra039753 gene encodes a plant homeodomain(PHD)-finger protein and is considered a potential candidate gene for Bra2Ms.Bra039753 was significantly downregulated in sterile line WS24-3 compared to the fertile line at the meiotic anther stage.Sequence analysis of Bra039753 identified a 369 bp fragment insertion in the first exon in male sterile plants,which led to an amino acid insertion in the Bra039753 protein.In addition,the 369 bp fragment insertion was found to cosegregate with the male sterility trait.This study identified a novel locus related to male sterility in non-heading Chinese cabbage,and the molecular marker obtained in this study will be beneficial for the marker-assisted selection of excellent sterile lines in non-heading Chinese cabbage and other Brassica crops.

non-heading Chinese cabbagemale sterilityBra2Msfine mappingPHD-finger protein

Liping Song、Xia Li、Liguang Tang、Chuying Yu、Bincai Wang、Changbin Gao、Yanfeng Xie、Xueli Zhang、Junliang Wang、Chufa Lin、Aihua Wang

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Wuhan Vegetable Research Institute,Wuhan Academy of Agricultural Sciences,Wuhan 430345,China

Biotechnology and Genetic Resources Institute,Yunnan Academy of Agricultural Sciences,Kunming 650000,China

Wuhan Major Project of Key Technologies in Biological Breeding and New Variety Cultivation,ChinaScience and Technology Support Project of Rural Vitalization in Hubei Province,China湖北省重点研发计划湖北省重点研发计划

20220213020248522022BBA1212021BBA0972021BBA102

2024

10.1016/j.jia.2023.08.008

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
年,卷(期)：2024.23(4)
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