首页|Development and formation of wing cuticle based on transcriptomic analysis in Locusta migratoria during metamorphosis
Development and formation of wing cuticle based on transcriptomic analysis in Locusta migratoria during metamorphosis
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国家科技期刊平台
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NSTL
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Wings are an important flight organ of insects.Wing development is a complex process controlled by a series of genes.The flightless wing pad transforms into a mature wing with the function of migratory flight during the nymph-to-adult metamorphosis.However,the mechanism of wing morphogenesis in locusts is still unclear.This study analyzed the microstructures of the locust wing pads at pre-eclosion and the wings after eclosion and performed the comparative transcriptome analysis.RNA-seq identified 25,334 unigenes and 3,430 differentially expressed genes(DEGs)(1,907 up-regulated and 1,523 down-regulated).The DEGs mainly included cuticle development(LmACPs),chitin metabolism(LmIdgf4),lipid metabolism-related genes,cell adhesion(Integrin),zinc finger transcription factors(LmSalm,LmZF593 and LmZF521),and others.Functional analysis based on RNA interference and hematoxylin and eosin(H&E)staining showed that the three genes encoded zinc finger transcription factors are essential for forming wing cuticle and maintaining morphology in Locusta migratoria.Finally,the study found that the LmSalm regulates the expression of LmACPs in the wing pads at pre-eclosion,and LmZF593 and LmZF521 regulate the expression of LmIntegrin/LmIdgf4/LmHMT420 in the wings after eclosion.This study revealed that the molecular regulatory axis controls wing morphology in nymphal and adult stages of locusts,offering a theoretical basis for the study of wing development mechanisms in hemimetabolous insects.
Shanxi Key Laboratory of Nucleic Acid Biopesticides,Institute of Applied Biology,Shanxi University,Taiyuan 030006,China
College of Life Science,Shanxi University,Taiyuan 030006,China
Guangmeiyuan R&D Center/Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology,South China Normal University,Meizhou 514779,China
国家重点研发计划国家自然科学基金earmarked fund for Modern Agroindustry Technology Research System,ChinaFund for Shanxi"1331 Project",ChinaScientific and Technological Innovation Programs of Higher Education Institutions in Shanxi,China
国家科技期刊平台
NSTL
万方数据
