农业科学学报(英文)2024，Vol.23Issue(4) ：1329-1337.DOI:10.1016/j.jia.2023.04.041

Responses of growth performance,antioxidant function,small intestinal morphology and mRNA expression of jejunal tight junction protein to dietary iron in yellow-feathered broilers

Kaiwen Lei Hao Wu Jerry W Spears Xi Lin Xi Wang Xue Bai Yanling Huang
农业科学学报(英文)2024，Vol.23Issue(4) ：1329-1337.DOI:10.1016/j.jia.2023.04.041
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Responses of growth performance,antioxidant function,small intestinal morphology and mRNA expression of jejunal tight junction protein to dietary iron in yellow-feathered broilers

Kaiwen Lei 1Hao Wu 1Jerry W Spears 2Xi Lin 2Xi Wang 1Xue Bai 1Yanling Huang1
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作者信息

  • 1. Key Laboratory of Animal Science of State Ethnic Affairs Commission,Southwest Minzu University,Chengdu 610041,China;Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization,Southwest Minzu University,Chengdu 610041,China
  • 2. Department of Animal Science,North Carolina State University,Raleigh,NC 27695-7621,USA
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Abstract

This study aimed to investigate the dose-effect of iron on growth performance,antioxidant function,intestinal morphology,and mRNA expression of jejunal tight junction protein in 1-to 21-d-old yellow-feathered broilers.A total of 720 1-d-old yellow-feathered male broilers were allocated to 9 treatments with 8 replicate cages of 10 birds per cage.The dietary treatments were consisted of a basal diet(contained 79.6 mg Fe kg-1)supplemented with 0,20,40,60,80,160,320,640,and 1,280 mg Fe kg-1 in the form of FeSO4·7H2O.Compared with the birds in the control group,birds supplemented with 20 mg Fe kg-1 had higher average daily gain(ADG)(P<0.0001).Adding 640 and 1,280 mg Fe kg-1 significantly decreased ADG(P<0.0001)and average daily feed intake(ADFI)(P<0.0001)compared with supplementation of 20 mg Fe kg-1.Malondialdehyde(MDA)concentration in plasma and duodenum increased linearly(P<0.0001),but MDA concentration in liver and jejunum increased linearly(P<0.05)or quadratically(P<0.05)with increased dietary Fe concentration.The villus height(VH)in duodenum and jejunum,and the ratio of villus height to crypt depth(V/C)in duodenum decreased linearly(P<0.05)as dietary Fe increased.As dietary Fe increased,the jejunal relative mRNA abundance of claudin-1 decreased linearly(P=0.001),but the jejunal relative mRNA abundance of zona occludens-1(ZO-1)and occludin decreased linearly(P<0.05)or quadratically(P<0.05).Compared with the supplementation of 20 mg Fe kg-1,the supplementation of 640 mg Fe kg-1 or higher increased(P<0.05)MDA concentrations in plasma,duodenum,and jejunum,decreased VH in the duodenum and jejunum,and the addition of 1,280 mg Fe kg-1 reduced(P<0.05)the jejunal tight junction protein(claudin-1,ZO-1,occludin)mRNA abundance.In summary,640 mg of supplemental Fe kg-1 or greater was associated with decreased growth performance,increased oxidative stress,disrupted intestinal morphology,and reduced mRNA expression of jejunal tight junction protein.

Key words

iron/yellow-feathered broiler/antioxidant function/intestinal morphology/tight junction protein

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基金项目

国家自然科学基金(31501977)

Sichuan Provincial Key Research and Development Project,China(22ZDYF0194)

Double World-Class Project of Southwest Minzu University,China(XM2023010)

出版年

2024
农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
参考文献量32
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