农业科学学报(英文)2024，Vol.23Issue(5) ：1481-1493.DOI:10.1016/j.jia.2023.06.020

A Golden2-like transcription factor,BnGLK1a,improves chloroplast development,photosynthesis,and seed weight in rapeseed

Qianwei Zhang Yuanyi Mao Zikun Zhao Xin Hu Ran Hu Nengwen Yin Xue Sun Fujun Sun Si Chen Yuxiang Jiang Liezhao Liu Kun Lu Jiana Li Yu Pan
农业科学学报(英文)2024，Vol.23Issue(5) ：1481-1493.DOI:10.1016/j.jia.2023.06.020
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A Golden2-like transcription factor,BnGLK1a,improves chloroplast development,photosynthesis,and seed weight in rapeseed

Qianwei Zhang 1Yuanyi Mao 1Zikun Zhao 1Xin Hu 2Ran Hu 1Nengwen Yin 1Xue Sun 1Fujun Sun 1Si Chen 1Yuxiang Jiang 1Liezhao Liu 1Kun Lu 1Jiana Li 1Yu Pan2
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作者信息

  • 1. Integrative Science Center of Germplasm Creation in Western China(Chongqing)Science City,Southwest University,Chongqing 400715,China;Academy of Agricultural Sciences,Southwest University,Chongqing 400715,China;Engineering Research Center of South Upland Agriculture,Ministry of Education,Chongqing 400715,China
  • 2. Academy of Agricultural Sciences,Southwest University,Chongqing 400715,China;Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education/College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China
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Abstract

Enhancing photosynthetic efficiency is a major goal for improving crop yields under agricultural field conditions and is associated with chloroplast biosynthesis and development.In this study,we demonstrate that Golden2-like 1a(BnGLK1a)plays an important role in regulating chloroplast development and photosynthetic efficiency.Overexpressing BnGLK1a resulted in significant increases in chlorophyll content,the number of thylakoid membrane layers and photosynthetic efficiency in Brassica napus,while knocking down BnGLK1a transcript levels through RNA interference(RNAi)had the opposite effects.A yeast two-hybrid screen revealed that BnGLK1a interacts with the abscisic acid receptor PYRABACTIN RESISTANCE 1-LIKE 1-2(BnPYL1-2)and CONSTITUTIVE PHOTOMORPHOGENIC 9 SIGNALOSOME 5A subunit(BnCSN5A),which play essential roles in regulating chloroplast development and photosynthesis.Consistent with this,BnGLK1a-RNAi lines of B.napus display hypersensitivity to the abscisic acid(ABA)response.Importantly,overexpression of BnGLK1a resulted in a 10％increase in thousand-seed weight,whereas seeds from BnGLK1a-RNAi lines were 16％lighter than wild type.We propose that BnGLK1a could be a potential target in breeding for improving rapeseed productivity.Our results not only provide insights into the mechanisms of BnGLK1a function,but also offer a potential approach for improving the productivity of Brassica species.

Key words

Brassica napus/BnGLK1a/chloroplast development/photosynthetic efficiency/yield

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基金项目

国家自然科学基金(32172597)

国家自然科学基金(31830067)

Chongqing Talents of Exceptional Young Talents Project,China(CQYC202005097)

Chongqing Talents of Exceptional Young Talents Project,China(cstc2021ycjhbgzxm0204)

Chongqing Talents of Exceptional Young Talents Project,China(cstc2021jcyjbshX0002)

China Agriculture Research System of MOF and MARA(CARS-12)

高等学校学科创新引智计划(111计划)(B12006)

Germplasm Creation Special Program of Southwest University,China()

出版年

2024
农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
参考文献量78
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