农业科学学报(英文)2024,Vol.23Issue(5) :1494-1506.DOI:10.1016/j.jia.2023.10.036

Fine-mapping of a candidate gene for web blotch resistance in Arachis hypogaea L.

Xiaohui Wu Mengyuan Zhang Zheng Zheng Ziqi Sun Feiyan Qi Hua Liu Juan Wang Mengmeng Wang Ruifang Zhao Yue Wu Xiao Wang Hongfei Liu Wenzhao Dong Xinyou Zhang
农业科学学报(英文)2024,Vol.23Issue(5) :1494-1506.DOI:10.1016/j.jia.2023.10.036

Fine-mapping of a candidate gene for web blotch resistance in Arachis hypogaea L.

Xiaohui Wu 1Mengyuan Zhang 2Zheng Zheng 3Ziqi Sun 3Feiyan Qi 3Hua Liu 3Juan Wang 3Mengmeng Wang 3Ruifang Zhao 3Yue Wu 3Xiao Wang 3Hongfei Liu 3Wenzhao Dong 3Xinyou Zhang4
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作者信息

  • 1. College of Agronomy,Henan Agricultural University,Zhengzhou 450046,China;Henan Academy of Agricultural Sciences/Henan Academy of Crop Molecular Breeding/Key Laboratory of Oil Crops in Huang-Huai-Hai Plain,Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crop Improvement,Zhengzhou 450002,China
  • 2. Shangqiu Academy of Agricultural and Forestry Sciences,Shangqiu 476000,China
  • 3. Henan Academy of Agricultural Sciences/Henan Academy of Crop Molecular Breeding/Key Laboratory of Oil Crops in Huang-Huai-Hai Plain,Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crop Improvement,Zhengzhou 450002,China
  • 4. Henan Academy of Agricultural Sciences/Henan Academy of Crop Molecular Breeding/Key Laboratory of Oil Crops in Huang-Huai-Hai Plain,Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crop Improvement,Zhengzhou 450002,China;The Shennong Laboratory,Zhengzhou 450002,China
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Abstract

Peanut(Arachis hypogaea L.)is a globally important oil crop.Web blotch is one of the most important foliar diseases affecting peanut,which results in serious yield losses worldwide.Breeding web blotch-resistant peanut varieties is the most effective and economically viable method for minimizing yield losses due to web blotch.In the current study,a bulked segregant analysis with next-generation sequencing was used to analyze an F2∶3 segregating population and identify candidate loci related to web blotch resistance.Based on the fine-mapping of the candidate genomic interval using kompetitive allele-specific PCR(KASP)markers,we identified a novel web blotch resistance-related locus spanning approximately 169 kb on chromosome 16.This region included four annotated genes,of which only Arahy.35VVQ3 had a non-synonymous single nucleotide polymorphism in the coding region between the two parents.Two markers(Chr.16.12872635 and Chr.16.12966357)linked to this gene were shown to be co-segregated with the resistance of peanut web blotch by 72 randomly selected recombinant inbred lines(RIL),which could be used in marker-assisted breeding of resistant peanut varieties.

Key words

peanut web blotch/bulked segregant analysis/KASP markers/resistant gene

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基金项目

Key Research Project of the Shennong Laboratory,China(SN01-2022-03)

Henan Provincial Science and Technology Research and Development Program Joint Fund(Superiority Discipline Cultivation)Proje(222301420100)

Major Science and Technology projects of Henan Province,China(221100110300)

China Agriculture Research System of MOF and MARA(CARS-13)

Henan Provincial Agriculture Research System,China(S2012-5)

Outstanding Young Scientists of Henan Academy of Agricultural Sciences,China(2022YQ16)

Independent Innovation Project of the Henan Academy of Agricultural Sciences,China(2023ZC093)

出版年

2024
农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子:0.576
ISSN:2095-3119
参考文献量55
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