首页|Fine-mapping of a candidate gene for web blotch resistance in Arachis hypogaea L.

Fine-mapping of a candidate gene for web blotch resistance in Arachis hypogaea L.

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  • 国家科技期刊平台
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Peanut(Arachis hypogaea L.)is a globally important oil crop.Web blotch is one of the most important foliar diseases affecting peanut,which results in serious yield losses worldwide.Breeding web blotch-resistant peanut varieties is the most effective and economically viable method for minimizing yield losses due to web blotch.In the current study,a bulked segregant analysis with next-generation sequencing was used to analyze an F2∶3 segregating population and identify candidate loci related to web blotch resistance.Based on the fine-mapping of the candidate genomic interval using kompetitive allele-specific PCR(KASP)markers,we identified a novel web blotch resistance-related locus spanning approximately 169 kb on chromosome 16.This region included four annotated genes,of which only Arahy.35VVQ3 had a non-synonymous single nucleotide polymorphism in the coding region between the two parents.Two markers(Chr.16.12872635 and Chr.16.12966357)linked to this gene were shown to be co-segregated with the resistance of peanut web blotch by 72 randomly selected recombinant inbred lines(RIL),which could be used in marker-assisted breeding of resistant peanut varieties.

peanut web blotchbulked segregant analysisKASP markersresistant gene

Xiaohui Wu、Mengyuan Zhang、Zheng Zheng、Ziqi Sun、Feiyan Qi、Hua Liu、Juan Wang、Mengmeng Wang、Ruifang Zhao、Yue Wu、Xiao Wang、Hongfei Liu、Wenzhao Dong、Xinyou Zhang

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College of Agronomy,Henan Agricultural University,Zhengzhou 450046,China

Henan Academy of Agricultural Sciences/Henan Academy of Crop Molecular Breeding/Key Laboratory of Oil Crops in Huang-Huai-Hai Plain,Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crop Improvement,Zhengzhou 450002,China

Shangqiu Academy of Agricultural and Forestry Sciences,Shangqiu 476000,China

The Shennong Laboratory,Zhengzhou 450002,China

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Key Research Project of the Shennong Laboratory,ChinaHenan Provincial Science and Technology Research and Development Program Joint Fund(Superiority Discipline Cultivation)ProjeMajor Science and Technology projects of Henan Province,ChinaChina Agriculture Research System of MOF and MARAHenan Provincial Agriculture Research System,ChinaOutstanding Young Scientists of Henan Academy of Agricultural Sciences,ChinaIndependent Innovation Project of the Henan Academy of Agricultural Sciences,China

SN01-2022-03222301420100221100110300CARS-13S2012-52022YQ162023ZC093

2024

10.1016/j.jia.2023.10.036

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
年,卷(期)：2024.23(5)
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