Fine-mapping of a candidate gene for web blotch resistance in Arachis hypogaea L.

Xiaohui Wu ¹Mengyuan Zhang ²Zheng Zheng ³Ziqi Sun ³Feiyan Qi ³Hua Liu ³Juan Wang ³Mengmeng Wang ³Ruifang Zhao ³Yue Wu ³Xiao Wang ³Hongfei Liu ³Wenzhao Dong ³Xinyou Zhang⁴

扫码查看

作者信息

1. College of Agronomy,Henan Agricultural University,Zhengzhou 450046,China;Henan Academy of Agricultural Sciences/Henan Academy of Crop Molecular Breeding/Key Laboratory of Oil Crops in Huang-Huai-Hai Plain,Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crop Improvement,Zhengzhou 450002,China
2. Shangqiu Academy of Agricultural and Forestry Sciences,Shangqiu 476000,China
3. Henan Academy of Agricultural Sciences/Henan Academy of Crop Molecular Breeding/Key Laboratory of Oil Crops in Huang-Huai-Hai Plain,Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crop Improvement,Zhengzhou 450002,China
4. Henan Academy of Agricultural Sciences/Henan Academy of Crop Molecular Breeding/Key Laboratory of Oil Crops in Huang-Huai-Hai Plain,Ministry of Agriculture and Rural Affairs/Henan Provincial Key Laboratory for Oil Crop Improvement,Zhengzhou 450002,China;The Shennong Laboratory,Zhengzhou 450002,China
折叠

Abstract

Peanut(Arachis hypogaea L.)is a globally important oil crop.Web blotch is one of the most important foliar diseases affecting peanut,which results in serious yield losses worldwide.Breeding web blotch-resistant peanut varieties is the most effective and economically viable method for minimizing yield losses due to web blotch.In the current study,a bulked segregant analysis with next-generation sequencing was used to analyze an F2∶3 segregating population and identify candidate loci related to web blotch resistance.Based on the fine-mapping of the candidate genomic interval using kompetitive allele-specific PCR(KASP)markers,we identified a novel web blotch resistance-related locus spanning approximately 169 kb on chromosome 16.This region included four annotated genes,of which only Arahy.35VVQ3 had a non-synonymous single nucleotide polymorphism in the coding region between the two parents.Two markers(Chr.16.12872635 and Chr.16.12966357)linked to this gene were shown to be co-segregated with the resistance of peanut web blotch by 72 randomly selected recombinant inbred lines(RIL),which could be used in marker-assisted breeding of resistant peanut varieties.

Key words

peanut web blotch/bulked segregant analysis/KASP markers/resistant gene

引用本文复制引用

基金项目

Key Research Project of the Shennong Laboratory,China(SN01-2022-03)

Henan Provincial Science and Technology Research and Development Program Joint Fund(Superiority Discipline Cultivation)Proje(222301420100)

Major Science and Technology projects of Henan Province,China(221100110300)

China Agriculture Research System of MOF and MARA(CARS-13)

Henan Provincial Agriculture Research System,China(S2012-5)

Outstanding Young Scientists of Henan Academy of Agricultural Sciences,China(2022YQ16)

Independent Innovation Project of the Henan Academy of Agricultural Sciences,China(2023ZC093)

出版年

2024

农业科学学报(英文)

中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD

影响因子：0.576

ISSN：2095-3119

参考文献量55

段落导航