EjGASA6 promotes flowering and root elongation by enhancing gibberellin biosynthesis

Qian Chen ¹Shunyuan Yong ¹Fan Xu ²Hao Fu ¹Jiangbo Dang ¹Qiao He ¹Danlong Jing ¹Di Wu ¹Guolu Liang ¹Qigao Guo¹

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作者信息

1. Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River(Ministry of Education)/College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400715,China;State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University/Academy of Agricultural Sciences of Southwest University,Chongqing 400715,China
2. College of Agronomy and Biotechnology,Southwest University,Chongqing 400715,China
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Abstract

The Gibberellic Acid-stimulated Arabidopsis(GASA)gene family is involved in the regulation of gene expression and plant growth,development,and stress responses.To investigate the function of loquat GASA genes in the growth and developmental regulation of plants,a loquat EjGASA6 gene homologous to Arabidopsis AtGASA6 was cloned.EjGASA6 expression was induced by gibberellin,and ectopic transgenic plants containing this gene exhibited earlier bloom and longer primary roots since these phenotypic characteristics are related to higher gibberellin content.Transcriptome analysis and qRT-PCR results showed that the expression levels of GA3ox1 and GA20ox1,which encode key enzymes in gibberellin biosynthesis,were significantly increased.Furthermore,we confirmed that EjGASA6 could promote the expression of GA20ox1 via the luciferase reporter system.Overall,our results suggest that EjGASA6 promotes blooming and main-root elongation by positively regulating gibberellin biosynthesis.These findings broaden our understanding of the role of GASAs in plant development and growth,and lay the groundwork for future research into the functions of EjGASA6 in regulating loquat growth and development.

Key words

loquat/EjGASA6/gibberellin synthesis/biological function

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基金项目

国家重点研发计划(2023YFD1600800)

国家自然科学基金(32102321)

Chongqing Science and Technology Commission,China(cstc2024ycjhbgzxm0202)

Chongqing Science and Technology Commission,China(cstc2021jscxgksbX0010)

Chongqing Science and Technology Commission,China(cstc2021jcyjmsxmX1156)

Chongqing Forestry Administration,China(YuLinKeYan2022-14)

Innovation Research Group Funds for Chongqing Universities,China(CXQT19005)

Characteristic Fruit Industry and Technology System Innovation Team of Chongqing Agriculture and Rural Affairs Commission,Ch(2022164)

Characteristic Fruit Industry and Technology System Innovation Team of Chongqing Agriculture and Rural Affairs Commission,Ch(2020301)

Chongqing Postgraduate Research and Innovation Programme,China(CYB23128)

出版年

2024

农业科学学报(英文)

中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD

影响因子：0.576

ISSN：2095-3119

参考文献量50

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