首页|Establishment of a system for screening and identification of novel bactericide targets in the plant pathogenic bacterium Xanthomonas oryzae pv.oryzae using Tn-seq and SPR

Establishment of a system for screening and identification of novel bactericide targets in the plant pathogenic bacterium Xanthomonas oryzae pv.oryzae using Tn-seq and SPR

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  • 国家科技期刊平台
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Xanthomonas spp.cause severe bacterial diseases.However,effective strategies for prevention and management of these diseases are scarce.Thus,it is necessary to improve the efficiency of control of diseases caused by Xanthomonas.In this study,Xanthomonas oryzae pv.oryzae(Xoo),which causes rice bacterial leaf blight,has been studied as a representative.A transposon insertion library of Xoo,comprising approximately 200,000 individual insertion mutants,was generated.Transposon sequencing data indicated that the mariner C9 transposase mapped at 35.7-36.4％of all potential insertion sites,revealing 491 essential genes required for the growth of Xoo in rich media.The results show that,compared to the functions of essential genes of other bacteria,the functions of some essential genes of Xoo are unknown,25 genes might be dangerous for the Xanthomonas group,and 3 are specific to Xanthomonas.High-priority candidates for developing broad-spectrum,Xanthomonas-specific,and environment-friendly bactericides were identified in this study.In addition,this study revealed the possible targets of dioctyldiethylenetriamine using surface plasmon resonance(SPR)in combination with high performance liquid chromatography-mass spectrometry(HPLC-MS).The study also provided references for the research of some certain bactericides with unknown anti-bacterial mode of action.In conclusion,this study urged a better understanding of Xanthomonas,provided meaningful data for the management of bacterial leaf blight,and disclosed selected targets of a novel bactericide.

rice bacterial leaf blightXanthomonasessential genesTn-seqdioctyldiethylenetriamine

Chaoyue Pang、Ling Jin、Haoyu Zang、Damalk Saint-Claire S.Koklannou、Jiazhi Sun、Jiawei Yang、Yongxing Wang、Liang Xu、Chunyan Gu、Yang Sun、Xing Chen、Yu Chen

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School of Plant Protection,Anhui Agricultural University,Hefei 230036,China

Anhui Province Laboratory for Green Pesticide Development and Application,School of Plant Protection,Anhui Agricultural University,Hefei 230036,China

Hefei Research Center,State Key Laboratory for Biology of Plant Diseases and Insect Pests,Hefei 230036,China

Key Laboratory of Integrated Crop Pest Management of Anhui Province,School of Plant Protection,Anhui Agricultural University,Hefei 230036,China

Institute of Plant Protection and Agro-Products Safety,Anhui Academy of Agricultural Sciences,Hefei 230031,China

Shandong Vicome Greenland Chemical Co.,Ltd.,Jinan 250204,China

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国家自然科学基金国家自然科学基金Programs for the Scientific Research Activities of Academic and Technical Leaders of Anhui Province,ChinaDevelopment Fund for Talent Personnel of Anhui Agricultural University,ChinaUniversity Synergy Innovation Program of Anhui Province,ChinaKey Project of the Natural Science Foundation of Anhui Provincial Department of Education,ChinaAnhui Province Agricultural Eco-Environmental Protection and Quality Safety Industry Technology System,China

32272587322023422020D251rc342006GXXT-2021-0592023AH040129

2024

10.1016/j.jia.2023.04.043

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
年,卷(期)：2024.23(5)
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