Functional assessment of cadherin as a shared mechanism for cross/dual resistance to Cry1Ac and Cry2Ab in Helicoverpa zea

Jizhen Wei ¹Min Zhang ²Pin Li ³Zhongyuan Deng ⁴Xinming Yin ³Shiheng An ³Xianchun Li⁵

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作者信息

1. State Key Laboratory of Wheat and Maize Crop Science/College of Plant Protection,Henan Agricultural University,Zhengzhou 450046,China;Department of Entomology and BI05 Institute,University of Arizona,Tucson,AZ 85721,USA
2. Department of Entomology and BI05 Institute,University of Arizona,Tucson,AZ 85721,USA;School of Agricultural Sciences,Zhengzhou University,Zhengzhou 450001,China
3. State Key Laboratory of Wheat and Maize Crop Science/College of Plant Protection,Henan Agricultural University,Zhengzhou 450046,China
4. School of Agricultural Sciences,Zhengzhou University,Zhengzhou 450001,China
5. Department of Entomology and BI05 Institute,University of Arizona,Tucson,AZ 85721,USA
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Abstract

Helicoverpa zea is a major target pest of pyramided transgenic crops expressing Cry1,Cry2 and/or Vip3Aa proteins from Bacillus thuringiensis(Bt)in the United States.Laboratory-selected Cry1Ac/Cry2Ab cross resistance and field-evolved practical dual resistance of H.zea to these two toxins have been widely reported.Whether the widespread Cry1 Ac/Cy2Ab dual resistance of H.zea has resulted from the selection of one shared or two independent resistance mechanisms by pyramided Bt crops remains unclear.Cadherin is a well-confirmed receptor of Cry1Ac and a suggested receptor of Cry2Ab in at least three Lepidopteran species.To test whether cadherin may serve as one shared mechanism for the cross and dual resistance of H.zea to Cry1Ac and Cry2Ab,we cloned H.zea cadherin(HzCadherin)cDNA and studied its functional roles in the mode of action of Cry1Ac and Cry2Ab by gain-and loss-of-function analyses.Heterologous expression of HzCadherin in H.zea midgut,H.zea fat body and Sf9 cells made all three of these cell lines more susceptible to activated Cry1Ac but not activated Cry2Ab,whereas silencing HzCadherin of H.zea midgut and fat body cells significantly reduced the susceptibility to Cry1Ac but not Cry2Ab.Likewise,suppressing HzCadherin with siRNA made H.zea larvae resistant to Cry1Ac.These results clearly demonstrate that HzCadherin is not a receptor for Cry2Ab,and thus it is unlikely to serve as one shared mechanism for the cross and dual resistance of H.zea to Cry1Ac and Cry2Ab.

Key words

Bt crops/cadherin/Cry toxin/receptor/resistance mechanism

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基金项目

USDA National Institute of Food and Agriculture(Hatch Grant ARZT-1360890-H31-164)

USDA National Institute of Food and Agriculture(multistate grant ARZT-1370680-R31-172NC246)

National Natural Science Foundation of China(NSFC)-Henan Joint Major Grant(U2004206)

State Key Laboratory of Cotton Biology Open Fund,Zhengzhou University,China(CB2020A06)

Henan Agriculture Research System,China(HARS-22-09-G3)

Earmarked Fund for China Agriculture Research System(CARS-27)

出版年

2024

农业科学学报(英文)

中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD

影响因子：0.576

ISSN：2095-3119

参考文献量92

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