首页|A novel live attenuated vaccine candidate protects chickens against subtype B avian metapneumovirus
A novel live attenuated vaccine candidate protects chickens against subtype B avian metapneumovirus
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国家科技期刊平台
NETL
NSTL
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Avian metapneumovirus(aMPV)is a highly contagious pathogen that causes acute upper respiratory tract diseases in chickens and turkeys,resulting in serious economic losses.Subtype B aMPV has recently become the dominant epidemic strain in China.We developed an attenuated aMPV subtype B strain by serial passaging in Vero cells and evaluated its safety and efficacy as a vaccine candidate.The safety test showed that after the 30th passage,the LN16-A strain was fully attenuated,as clinical signs of infection and histological lesions were absent after inoculation.The LN16-A strain did not revert to a virulent strain after five serial passages in chickens.The genomic sequence of LN16-A differed from that of the parent wild-type LN16(wtLN16)strain and had nine amino acid mutations.In chickens,a single immunization with LN16-A induced robust humoral and cellular immune responses,including the abundant production of neutralizing antibodies,CD4+T lymphocytes,and the Th1(IFN-y)and Th2(IL-4 and IL-6)cytokines.We also confirmed that LN16-A provided 100％protection against subtype B aMPV and significantly reduced viral shedding and turbinate inflammation.Our findings suggest that the LN16-A strain is a promising live attenuated vaccine candidate that can prevent infection with subtype B aMPV.
Avian Immunosuppressive Diseases Division,State Key Laboratory for Animal Disease Control and Prevention,Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Harbin 150069,China
National Poultry Laboratory Animal Resource Center,Harbin 150069,China
国家重点研发计划China Agriculture Research SystemHeilongjiang Touyan Innovation Team Program of China
国家科技期刊平台
NSTL
万方数据
