Duplicated chaicone synthase(CHS)genes modulate flavonoid production in tea plants in response to light stress

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外文摘要：In tea plants,the abundant flavonoid compounds are responsible for the health benefits for the human body and define the astringent flavor profile.While the downstream mechanisms of flavonoid biosynthesis have been extensively studied,the role of chalcone synthase(CHS)in this secondary metabolic process in tea plants remains less clear.In this study,we compared the evolutionary profile of the flavonoid metabolism pathway and discovered that gene duplication of CHS occurred in tea plants.We identified three CsCHS genes,along with a CsCHS-like gene,as potential candidates for further functional investigation.Unlike the CsCHS-like gene,the CsCHS genes effectively restored flavonoid production in Arabidopsis chs-mutants.Additionally,CsCHS transgenic tobacco plants exhibited higher flavonoid compound accumulation compared to their wild-type counterparts.Most notably,our examination of promoter and gene expression levels for the selected CHS genes revealed distinct responses to UV-B stress in tea plants.Our findings suggest that environmental factors such as UV-B exposure could have been the key drivers behind the gene duplication events in CHS.

作者：

Mingzhuo Li、Wenzhao Wang、Yeru Wang、Lili Guo、Yajun Liu、Xiaolan Jiang、Liping Gao、Tao Xia

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作者单位：

State Key Laboratory of Tea Plant Biochemistry and Utilization,Anhui Agricultural University,Hefei 230036,China

Department of Plant and Microbial Biology,North Carolina State University,Raleigh 27606,NC,USA

College of Horticulture,Northwest A&F University,Yangling 712100,China

School of Life Sciences,Anhui Agricultural University,Hefei 230036,China

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基金：

国家自然科学基金国家自然科学基金国家自然科学基金

项目编号：

U21A202323237275632202551

出版年：

2024

DOI：

10.1016/j.jia.2024.03.060

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农业科学学报(英文)

中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD

影响因子：0.576

ISSN：2095-3119

年,卷(期)：2024.23(6)

Mingzhuo Li,Wenzhao Wang,Yeru Wang,et al.Duplicated chaicone synthase(CHS)genes modulate flavonoid production in tea plants in response to light stress[J].农业科学学报(英文),2024,23(6):1940-1955.DOI:10.1016/j.jia.2024.03.060.

参考文献量63