The DNA damage repair complex MoMMS21-MoSMC5 is required for infection-related development and pathogenicity of Magnaporthe oryzae

Yue Jiang ¹Rong Wang ²Lili Du ³Xueyu Wang ²Xi Zhang ²Pengfei Qi ³Qianfei Wu ³Baoyi Peng ³Zonghua Wang ⁴Mo Wang ⁵Ya Li⁶

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作者信息

1. State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,Fujian Agriculture and Forestry University,Fuzhou 350002,China;State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Yunnan Agricultural University,Kunming 650201,China
2. Fujian Universities Key Laboratory for Plant-Microbe Interaction,Fujian Agriculture and Forestry University,Fuzhou 350002,China
3. State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,Fujian Agriculture and Forestry University,Fuzhou 350002,China
4. State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fujian Universities Key Laboratory for Plant-Microbe Interaction,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops,Minjiang University,Fuzhou 350108,China
5. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Yunnan Agricultural University,Kunming 650201,China
6. State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fujian Universities Key Laboratory for Plant-Microbe Interaction,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests,Fuzhou 350003,China
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Abstract

The conserved DNA damage repair complex,MMS21-SMC5/6(Methyl methane sulfonate 21-Structural maintenance of chromosomes 5/6),has been extensively studied in yeast,animals,and plants.However,its role in phytopathogenic fungi,particularly in the highly destructive rice blast fungus Magnaporthe oryzae,remains unknown.In this study,we functionally characterized the homologues of this complex,MoMMS21 and MoSMC5,in M.oryzae.We first demonstrated the importance of DNA damage repair in M.oryzae by showing that the DNA damage inducer phleomycin inhibited vegetative growth,infection-related development and pathogenicity in this fungus.Additionally,we discovered that MoMMS21 and MoSMC5 interacted in the nuclei,suggesting that they also function as a complex in M.oryzae.Gene deletion experiments revealed that both MoMMS21 and MoSMC5 are required for infection-related development and pathogenicity in M.oryzae,while only MoMMS21 deletion affected growth and sensitivity to phleomycin,indicating its specific involvement in DNA damage repair.Overall,our results provide insights into the roles of MoMMS21 and MoSMC5 in M.oryzae,highlighting their functions beyond DNA damage repair.

Key words

Magnaporthe oryzae/MMS21/SMC5/DNA damage repair/pathogenicity

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基金项目

国家重点研发计划(2023YFD1400200)

福建省自然科学基金(2022J01125)

Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests,China(MIMCP-202301)

Fujian Provincial Science and Technology Key Project,China(2022NZ030014)

国家自然科学基金(NSFC31871914)

出版年

2024

农业科学学报(英文)

中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD

影响因子：0.576

ISSN：2095-3119

参考文献量2

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