首页|The DNA damage repair complex MoMMS21-MoSMC5 is required for infection-related development and pathogenicity of Magnaporthe oryzae

The DNA damage repair complex MoMMS21-MoSMC5 is required for infection-related development and pathogenicity of Magnaporthe oryzae

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The conserved DNA damage repair complex,MMS21-SMC5/6(Methyl methane sulfonate 21-Structural maintenance of chromosomes 5/6),has been extensively studied in yeast,animals,and plants.However,its role in phytopathogenic fungi,particularly in the highly destructive rice blast fungus Magnaporthe oryzae,remains unknown.In this study,we functionally characterized the homologues of this complex,MoMMS21 and MoSMC5,in M.oryzae.We first demonstrated the importance of DNA damage repair in M.oryzae by showing that the DNA damage inducer phleomycin inhibited vegetative growth,infection-related development and pathogenicity in this fungus.Additionally,we discovered that MoMMS21 and MoSMC5 interacted in the nuclei,suggesting that they also function as a complex in M.oryzae.Gene deletion experiments revealed that both MoMMS21 and MoSMC5 are required for infection-related development and pathogenicity in M.oryzae,while only MoMMS21 deletion affected growth and sensitivity to phleomycin,indicating its specific involvement in DNA damage repair.Overall,our results provide insights into the roles of MoMMS21 and MoSMC5 in M.oryzae,highlighting their functions beyond DNA damage repair.

Magnaporthe oryzaeMMS21SMC5DNA damage repairpathogenicity

Yue Jiang、Rong Wang、Lili Du、Xueyu Wang、Xi Zhang、Pengfei Qi、Qianfei Wu、Baoyi Peng、Zonghua Wang、Mo Wang、Ya Li

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State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,Fujian Agriculture and Forestry University,Fuzhou 350002,China

State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan,Yunnan Agricultural University,Kunming 650201,China

Fujian Universities Key Laboratory for Plant-Microbe Interaction,Fujian Agriculture and Forestry University,Fuzhou 350002,China

Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops,Minjiang University,Fuzhou 350108,China

Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests,Fuzhou 350003,China

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国家重点研发计划福建省自然科学基金Fujian Key Laboratory for Monitoring and Integrated Management of Crop Pests,ChinaFujian Provincial Science and Technology Key Project,China国家自然科学基金

2023YFD14002002022J01125MIMCP-2023012022NZ030014NSFC31871914

2024

10.1016/j.jia.2024.01.010

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
年,卷(期)：2024.23(6)
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