首页|A bacterial protein Rhp-PSP inhibits plant viral proliferation through endoribonuclease activity

A bacterial protein Rhp-PSP inhibits plant viral proliferation through endoribonuclease activity

扫码查看
原文链接
  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
Plant virus causes massive crop losses globally.However,there is currently no effective measure to control plant viral disease.Previously,we identify an antiviral protein Rhp-PSP,produced by the bacterial Rhodopseudomonas palustris strain JSC-3b.In this study,we discover that the antiviral activity of Rhp-PSP relies on its endoribonuclease activity.Converting the arginine(R)residue at position 129 onto alanine(A)abolishs its endoribonuclease activity on coat protein(CP)RNA of tobacco mosaic virus(TMV),consequentially,compromises the antiviral activity of Rhp-PSP.Further investigation demonstrates that,the mutant Rhp-PSPR129A is unable to form the homotrimer as the wild type,indicating the importance of quaternary junction for the endoribonuclease activity.Overexpression of Rhp-PSP in Nicotiana benthamiana significantly enhances the resistance against TMV of seedlings,while expression of Rhp-PSPR129Adid not,confirming that endoribonuclease activity is responsible for the antiviral activity of Rhp-PSP.In addition,foliar spray of Rhp-PSP solution on tomato and pepper plants significantly reduces the disease index of viral diseases,indicating that Rhp-PSP shows potential to develop antiviral agent in practice.

Rhodopseudomonas palustrisRhp-PSPtobacco mosaic virusendoribonucleaseantiviral activity

Lijie Chen、Qianze Peng、Xiaohua Du、Weixing Zhang、Ju'e Cheng、Shu'e Sun、Deyong Zhang、Pin Su、Yong Liu

展开 >

Longping Branch,College of Biology,Hunan University,Changsha 410082,China

State Key Laboratory of Hybrid Rice/Institute of Plant Protection,Hunan Academy of Agricultural Sciences,Changsha 410125,China

国家重点研发计划湖南省重点研发计划Hunan Natural Science Foundation,ChinaAgricultural Science and Technology Innovation Fund Project of Hunan Province,ChinaChangsha Natural Science Foundation,China

2022YFD14007002022NK20142022JJ402342022CX1kq2202338

2024

10.1016/j.jia.2024.03.051

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
年,卷(期)：2024.23(6)
  • 42