首页|Eureka lemon zinc finger protein CIDOF3.4 interacts with citrus yellow vein clearing virus coat protein to inhibit viral infection
Eureka lemon zinc finger protein CIDOF3.4 interacts with citrus yellow vein clearing virus coat protein to inhibit viral infection
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国家科技期刊平台
NETL
NSTL
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Citrus yellow vein clearing virus(CYVCV)is a new citrus virus that has become an important factor restricting the development of China's citrus industry,and the CYVCV coat protein(CP)is associated with viral pathogenicity.In this study,the Eureka lemon zinc finger protein(ZFP)CIDOF3.4 was shown to interact with CYVCV CP in vivo and in vitro.Transient expression of CIDOF3.4 in Eureka lemon induced the expression of salicylic acid(SA)-related and hypersensitive response marker genes,and triggered a reactive oxygen species burst,ion leakage necrosis,and the accumulation of free SA.Furthermore,the CYVCV titer in CIDOF3.4 transgenic Eureka lemon plants was approximately 69.4％that in control plants 6 mon after inoculation,with only mild leaf chlorotic spots observed in those transgenic plants.Taken together,the results indicate that CIDOF3.4 not only interacts with CP but also induces an immune response in Eureka lemon by inducing the SA pathways.This is the first report that ZFP is involved in the immune response of a citrus viral disease,which provides a basis for further study of the molecular mechanism of CYVCV infection.
Integrative Science Center of Germplasm Creation in Western China(CHONGQING)Science City,Citrus Research Institute,Southwest University,Chongqing 400712,China
National Citrus Engineering Research Center,Citrus Research Institute,Southwest University,Chongqing 400712,China
Chongqing Three Gorges Academy of Agricultural Science,Chongqing Three Gorges University,Chongqing 404020,China
China Agriculture Research System of MOF and MARAInnovation Research Pilot Plan of Southwest University,China(2035)Guangxi Science and Technology Planed Project,China广西自然科学基金
CARS-26-05BSWU-XDPY22002Gui Ke AD230260902023GXNSFBA026285
国家科技期刊平台
NSTL
万方数据
