Abstract
Citrus yellow vein clearing virus(CYVCV)is a new citrus virus that has become an important factor restricting the development of China's citrus industry,and the CYVCV coat protein(CP)is associated with viral pathogenicity.In this study,the Eureka lemon zinc finger protein(ZFP)CIDOF3.4 was shown to interact with CYVCV CP in vivo and in vitro.Transient expression of CIDOF3.4 in Eureka lemon induced the expression of salicylic acid(SA)-related and hypersensitive response marker genes,and triggered a reactive oxygen species burst,ion leakage necrosis,and the accumulation of free SA.Furthermore,the CYVCV titer in CIDOF3.4 transgenic Eureka lemon plants was approximately 69.4％that in control plants 6 mon after inoculation,with only mild leaf chlorotic spots observed in those transgenic plants.Taken together,the results indicate that CIDOF3.4 not only interacts with CP but also induces an immune response in Eureka lemon by inducing the SA pathways.This is the first report that ZFP is involved in the immune response of a citrus viral disease,which provides a basis for further study of the molecular mechanism of CYVCV infection.
基金项目
China Agriculture Research System of MOF and MARA(CARS-26-05B)
Innovation Research Pilot Plan of Southwest University,China(2035)(SWU-XDPY22002)
Guangxi Science and Technology Planed Project,China(Gui Ke AD23026090)
广西自然科学基金(2023GXNSFBA026285)
国家科技期刊平台
NSTL
万方数据