农业科学学报(英文)2024,Vol.23Issue(7) :2155-2163.DOI:10.1016/j.jia.2023.07.026

Genetic analysis and fine mapping of a grain size QTL in the small-grain sterile rice line Zhuo201S

Bin Lei Jiale Shao Feng Zhang Jian Wang Yunhua Xiao Zhijun Cheng Wenbang Tang Jianmin Wan
农业科学学报(英文)2024,Vol.23Issue(7) :2155-2163.DOI:10.1016/j.jia.2023.07.026

Genetic analysis and fine mapping of a grain size QTL in the small-grain sterile rice line Zhuo201S

Bin Lei 1Jiale Shao 2Feng Zhang 2Jian Wang 2Yunhua Xiao 3Zhijun Cheng 2Wenbang Tang 4Jianmin Wan5
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作者信息

  • 1. College of Agronomy,Hunan Agricultural University,Changsha 410128,China;National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • 2. National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China
  • 3. College of Agronomy,Hunan Agricultural University,Changsha 410128,China
  • 4. College of Agronomy,Hunan Agricultural University,Changsha 410128,China;State Key Laboratory of Hybrid Rice,Hunan Hybrid Rice Research Center,Changsha 410125,China
  • 5. National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China;State Key Laboratory for Crop Genetics and Germplasm Enhancement/Jiangsu Plant Gene Engineering Research Center,Nanjing Agricultural University,Nanjing 210095,China
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Abstract

The development and application of the small-grain rice sterile line Zhuo201S(Z201S)has demonstrated its potential for mechanized hybrid rice seed production,leading to significant cost reductions.However,the molecular mechanism responsible for the small-grain size characteristic of Z201S remains unclear.In this study,we conducted a genetic analysis using near-isogenic lines constructed from Z210S,a small-grain rice sterile line,and R2115,a normal-grain variety.The results revealed that the small-grain trait in Z201S is governed by a single partially dominant gene which also enhances grain number.Through mapping,we localized the causal gene to the short arm of chromosome 2,within a 113 kb physical region delimited by the molecular markers S2-4-1 and LB63.Transgenic analysis and gene expression assays indicated LOC_Os02g14760 as the most likely candidate gene,suggesting that the small-grain size trait of Z201S is controlled by a novel locus that has not been previously identified.

Key words

rice/grain size/map-based cloning

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基金项目

National Natural Science Foundation of China(32172078)

National Natural Science Foundation of China(U22A20502)

出版年

2024
农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子:0.576
ISSN:2095-3119
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