首页|Genetic analysis and fine mapping of a grain size QTL in the small-grain sterile rice line Zhuo201S
Genetic analysis and fine mapping of a grain size QTL in the small-grain sterile rice line Zhuo201S
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国家科技期刊平台
NETL
NSTL
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The development and application of the small-grain rice sterile line Zhuo201S(Z201S)has demonstrated its potential for mechanized hybrid rice seed production,leading to significant cost reductions.However,the molecular mechanism responsible for the small-grain size characteristic of Z201S remains unclear.In this study,we conducted a genetic analysis using near-isogenic lines constructed from Z210S,a small-grain rice sterile line,and R2115,a normal-grain variety.The results revealed that the small-grain trait in Z201S is governed by a single partially dominant gene which also enhances grain number.Through mapping,we localized the causal gene to the short arm of chromosome 2,within a 113 kb physical region delimited by the molecular markers S2-4-1 and LB63.Transgenic analysis and gene expression assays indicated LOC_Os02g14760 as the most likely candidate gene,suggesting that the small-grain size trait of Z201S is controlled by a novel locus that has not been previously identified.
ricegrain sizemap-based cloning
Bin Lei、Jiale Shao、Feng Zhang、Jian Wang、Yunhua Xiao、Zhijun Cheng、Wenbang Tang、Jianmin Wan
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College of Agronomy,Hunan Agricultural University,Changsha 410128,China
National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China
State Key Laboratory of Hybrid Rice,Hunan Hybrid Rice Research Center,Changsha 410125,China
State Key Laboratory for Crop Genetics and Germplasm Enhancement/Jiangsu Plant Gene Engineering Research Center,Nanjing Agricultural University,Nanjing 210095,China
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National Natural Science Foundation of ChinaNational Natural Science Foundation of China
国家科技期刊平台
NSTL
万方数据
