Identification of P-type plasma membrane H+-ATPases in common wheat and characterization of TaHA7 associated with seed dormancy and germination

Bingli Jiang ¹Wei Gao ¹Yating Jiang ¹Shengnan Yan ¹Jiajia Cao ¹Litian Zhang ¹Yue Zhang ¹Jie Lu ¹Chuanxi Ma ¹Cheng Chang ¹Haiping Zhang¹

扫码查看

作者信息

1. Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow & Huai River Valley,Ministry of Agriculture and Rural Affairs/College of Agronomy,Anhui Agricultural University,Hefei 230036,China
折叠

Abstract

The P-type plasma membrane(PM)H+-ATPases(HAs)are crucial for plant development,growth,and defense.The HAs have been thoroughly characterized in many different plants.However,despite their importance,the functions of HAs in germination and seed dormancy(SD)have not been validated in wheat.Here,we identified 28 TaHA genes(TaHA1-28)in common wheat,which were divided into five subfamilies.An examination of gene expression in strong-and weak-SD wheat varieties led to the discovery of six candidate genes(TaHA7/-12/-14/-16/-18/-20).Based on a single nucleotide polymorphism(SNP)mutation(C/T)in the TaHA7 coding region,a CAPS marker(HA7)was developed and validated in 168 wheat varieties and 171 Chinese mini-core collections that exhibit diverse germination and SD phenotypes.We further verified the roles of the two allelic variations of TaHA7 in germination and SD using wheat mutants mutagenized with ethyl methane sulphonate(EMS)in'Jimai 22'and'Jing 411'backgrounds,and in transgenic Arabidopsis lines.TaHA7 appears to regulate germination and SD by mediating gibberellic acid(GA)and abscisic acid(ABA)signaling,metabolism,and biosynthesis.The results presented here will enable future research regarding the TaHAs in wheat.

Key words

wheat/P-type plasma membrane H+-ATPase/seed dormancy/abscisic acid/gibberellin

引用本文复制引用

基金项目

University Synergy Innovation Program of Anhui Province,China(GXXT-2021-058)

National Natural Science Foundation of China(U20A2033)

Natural Science Foundation of Anhui Province,China(2108085MC98)

Key Scientific and Technological Breakthroughs of Anhui Province,China(2021d06050003)

Anhui Province Education Department Sciences Research Project,China(YJS20210212)

Scientific Research Project of Higher Education in Anhui Province,China(2022AH050924)

Scientific Research Project of Higher Education in Anhui Province,China(2022AH050885)

Jiangsu Collaborative Innovation Center for Modern Crop Production,China(JCIC-MCP)()

出版年

2024

农业科学学报(英文)

中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD

影响因子：0.576

ISSN：2095-3119

段落导航