首页|Identification of P-type plasma membrane H+-ATPases in common wheat and characterization of TaHA7 associated with seed dormancy and germination

Identification of P-type plasma membrane H+-ATPases in common wheat and characterization of TaHA7 associated with seed dormancy and germination

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  • 国家科技期刊平台
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The P-type plasma membrane(PM)H+-ATPases(HAs)are crucial for plant development,growth,and defense.The HAs have been thoroughly characterized in many different plants.However,despite their importance,the functions of HAs in germination and seed dormancy(SD)have not been validated in wheat.Here,we identified 28 TaHA genes(TaHA1-28)in common wheat,which were divided into five subfamilies.An examination of gene expression in strong-and weak-SD wheat varieties led to the discovery of six candidate genes(TaHA7/-12/-14/-16/-18/-20).Based on a single nucleotide polymorphism(SNP)mutation(C/T)in the TaHA7 coding region,a CAPS marker(HA7)was developed and validated in 168 wheat varieties and 171 Chinese mini-core collections that exhibit diverse germination and SD phenotypes.We further verified the roles of the two allelic variations of TaHA7 in germination and SD using wheat mutants mutagenized with ethyl methane sulphonate(EMS)in'Jimai 22'and'Jing 411'backgrounds,and in transgenic Arabidopsis lines.TaHA7 appears to regulate germination and SD by mediating gibberellic acid(GA)and abscisic acid(ABA)signaling,metabolism,and biosynthesis.The results presented here will enable future research regarding the TaHAs in wheat.

wheatP-type plasma membrane H+-ATPaseseed dormancyabscisic acidgibberellin

Bingli Jiang、Wei Gao、Yating Jiang、Shengnan Yan、Jiajia Cao、Litian Zhang、Yue Zhang、Jie Lu、Chuanxi Ma、Cheng Chang、Haiping Zhang

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Key Laboratory of Wheat Biology and Genetic Improvement on Southern Yellow & Huai River Valley,Ministry of Agriculture and Rural Affairs/College of Agronomy,Anhui Agricultural University,Hefei 230036,China

University Synergy Innovation Program of Anhui Province,ChinaNational Natural Science Foundation of ChinaNatural Science Foundation of Anhui Province,ChinaKey Scientific and Technological Breakthroughs of Anhui Province,ChinaAnhui Province Education Department Sciences Research Project,ChinaScientific Research Project of Higher Education in Anhui Province,ChinaScientific Research Project of Higher Education in Anhui Province,ChinaJiangsu Collaborative Innovation Center for Modern Crop Production,China(JCIC-MCP)

GXXT-2021-058U20A20332108085MC982021d06050003YJS202102122022AH0509242022AH050885

2024

10.1016/j.jia.2023.07.023

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
年,卷(期)：2024.23(7)