Quantitative trait loci identification reveals zinc finger protein CONSTANS-LIKE 4 as the key candidate gene of stigma color in watermelon(Citrullus lanatus)

Shuang Pei ¹Zexu Wu ¹Ziqiao Ji ¹Zheng Liu ²Zicheng Zhu ¹Feishi Luan ¹Shi Liu¹

扫码查看

作者信息

1. Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region),Ministry of Agriculture and Rural Affairs/College of Horticulture and Landscape Architecture,Northeast Agricultural University,Harbin 150030,China
2. Institute of Rural Revitalization Science and Technology,Heilongjiang Academy of Agricultural Sciences,Harbin 150028,China
折叠

Abstract

Stigma color is a critical agronomic trait in watermelon that plays an important role in pollination.However,there are few reports on the regulation of stigma color in watermelon.In this study,a genetic analysis of the F2 population derived from ZXG1553(P1,with orange stigma)and W1-17(P2,with yellow stigma)indicated that stigma color is a quantitative trait and the orange stigma is recessive compared with the yellow stigma.Bulk segregant analysis sequencing(BSA-seq)revealed a 3.75 Mb segment on chromosome 6 that is related to stigma color.Also,a major stable effective QTL Clqsc6.1(QTL stigma color)was detected in two years between cleaved amplified polymorphic sequencing(CAPS)markers Chr06_8338913 and Chr06_9344593 spanning a～1.01 Mb interval that harbors 51 annotated genes.Cla97C06G117020(annotated as zinc finger protein CONSTANS-LIKE 4)was identified as the best candidate gene for the stigma color trait through RNA-seq,quantitative real-time PCR(qRT-PCR),and gene structure alignment analysis among the natural watermelon panel.The expression level of Cla97C06G117020 in the orange stigma accession was lower than in the yellow stigma accessions with a significant difference.A nonsynonymous SNP site of the Cla97C06G117020 coding region that causes amino acid variation was related to the stigma color variation among nine watermelon accessions according to their re-sequencing data.Stigma color formation is often related to carotenoids,and we also found that the expression trend of CICHYB(annotated as β-carotene hydroxylase)in the carotenoid metabolic pathway was consistent with Cla97C06G117020,and it was expressed in low amounts in the orange stigma accession.These data indicated that Cla97C06G117020 and CICHYB may interact to form the stigma color.This study provides a theoretical basis for gene fine mapping and mechanisms for the regulation of stigma color in watermelon.

Key words

watermelon/stigma color/gene mapping/zinc finger protein CONSTANS-LIKE 4

引用本文复制引用

基金项目

Natural Science Funds for Outstanding Youth of Heilongjiang Province,China(YQ2022C011)

National Natural Science Foundation of China(32172577)

China Agriculture Research System of MOF and MARA,China(CARS-25)

Taishan Industrial Leading Talents Project,China(LJNY202112)

Natural Science Foundation of Heilongjiang Province,China(LH2022C025)

出版年

2024

农业科学学报(英文)

中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD

影响因子：0.576

ISSN：2095-3119

段落导航