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Development of a stable attenuated double-mutant of tobacco mosaic virus for cross-protection

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Tobacco(Nicotiana tabacum)and tomato(Solanum lycopersicum)are two major economic crops in China.Tobacco mosaic virus(TMV;genus Tobamovirus)is the most prevalent virus infecting both crops.Currently,some widely cultivated tobacco and tomato cultivars are susceptible to TMV and there is no effective strategy to control this virus.Cross-protection can be a safe and environmentally friendly strategy to prevent viral diseases.However,stable attenuated TMV mutants are scarce.In this study,we found that the substitutions in the replicase p126,arginine at position 196(R196)with aspartic acid(D),glutamic acid at position 614(E614)with glycine(G),serine at position 643(S643)with phenylalanine(F),or D at position 730(D730)with S,significantly reduced the virulence and replication of TMV.However,only the mutation of S643 to F reduced the RNA silencing suppression activity of TMV p126.A double-mutant TMV-E614G-S643F induced no visible symptom and was genetically stable through six successive passages in tobacco plants.Furthermore,our results showed that TMV-E614G-S643F double-mutant could provide effective protection against the wild-type TMV infection in tobacco and tomato plants.This study reports a promising mild mutant for cross-protection to control TMV in tobacco and tomato plants.

cross-protectiondouble-mutantp126tobacco mosaic virusvirulence

Xiaojie Xu、Shaoyan Jiang、Chunju Liu、Xujie Sun、Qing Zhu、Xiuzhai Chen、Pengchao Jiang、Fenglong Wang、Yanping Tian、Xiangdong Li

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The Engineering Research Institute of Agriculture and Forestry,Ludong University,Yantai 264025,China

Laboratory of Plant Virology,Department of Plant Pathology,College of Plant Protection,Shandong Agricultural University,Tai'an 271018,China

Weifang Tobacco Corporation,Weifang 261031,China

Linyi Tobacco Corporation,Linyi 276000,China

Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao 266101,China

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'Taishan Scholar'Construction Project,China'Taishan Scholar'Construction Project,China

TS2022-028202101-KN275

2024

10.1016/j.jia.2024.02.019

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
年,卷(期)：2024.23(7)