首页|Dynamic transcriptome profiles and novel markers in bovine spermatogenesis revealed by single-cell sequencing

Dynamic transcriptome profiles and novel markers in bovine spermatogenesis revealed by single-cell sequencing

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
Testicular development is an important biological process in male and requires interaction between the male germ cells and somatic cells.However,the mechanisms of testicular development in livestock,particularly in cattle,are poorly understood.Furthermore,cellular heterogeneity hinders the profiling of different cell types at different developmental stages.In this study,we first performed a single-cell transcriptomic study of the bovine testis development during puberty by using 10× genomics single-cell RNA sequencing(scRNA-seq).By collecting the scRNA-seq data from 11,083 cells from prepubertal and pubertal bovine testes,a high-resolution scRNA-seq atlas was described,identifying 9 somatic and 13 spermatogenic clusters.We also distinguished several stage-specific marker genes for bovine germ cells and somatic cells,such as GRAF2 and MORC1 for SSC(spermatogonial stem cells),HJURP and TCF19 for differentiating spermatogonia,ARSE for immature Sertoli,CLEC12B for mature Sertoli,LOC112441470 for Leydig.In conclusion,we have examined the transcription levels and constructed the single-cell developmental maps of germ cells and somatic cells during testicular development in Angus cattle.The datasets provided new insights into spermatogenesis and testicular somatic cell development in cattle.

scRNA-seqcattlespermatogenesistestis development

Yuan Gao、Fuxia Bai、Qi Zhang、Xiaoya An、Zhaofei Wang、Chuzhao Lei、Ruihua Dang

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Key Laboratory of Animal Genetics,Breeding and Reproduction of Shaanxi Province,College of Animal Science and Technology,Northwest A&F University,Yangling 712100,China

College of Life Sciences,Northwestern Polytechnical University,Xi'an 710072,China

National Natural Science Foundation of China2020 Scientist Plus Engineer Program of Shaanxi Province,ChinaKey Research & Development Plan of Shaanxi Province,ChinaNatural Science Basis Research Plan of Shaanxi Province,ChinaChina Agriculture Research System of MOF and MARA

81770514K40504224682020NY-0022023-JC-QN-0242CARS-37

2024

10.1016/j.jia.2023.04.036

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
年,卷(期)：2024.23(7)