农业科学学报(英文)2024，Vol.23Issue(7) ：2379-2390.DOI:10.1016/j.jia.2023.04.014

Asymmetric expression of CA2 and CA13 linked to calcification in the bilateral mandibular condyles cause crossed beaks in chickens

Lei Shi Yanyan Sun Yunlei Li Hao Bai Jingwei Yuan Hui Ma Yuanmei Wang Panlin Wang Aixin Ni Linlin Jiang Pingzhuang Ge Shixiong Bian Yunhe Zong Jinmeng Zhao Adamu M.Isa Hailai H.Tesfay Jilan Chen
农业科学学报(英文)2024，Vol.23Issue(7) ：2379-2390.DOI:10.1016/j.jia.2023.04.014
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Asymmetric expression of CA2 and CA13 linked to calcification in the bilateral mandibular condyles cause crossed beaks in chickens

Lei Shi 1Yanyan Sun 2Yunlei Li 2Hao Bai 3Jingwei Yuan 2Hui Ma 2Yuanmei Wang 2Panlin Wang 2Aixin Ni 2Linlin Jiang 2Pingzhuang Ge 2Shixiong Bian 2Yunhe Zong 2Jinmeng Zhao 2Adamu M.Isa 2Hailai H.Tesfay 2Jilan Chen2
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作者信息

  • 1. State Key Laboratory of Animal Biotech Breeding/Key Laboratory of Animal(Poultry)Genetics Breeding and Reproduction of Ministry of Agriculture and Rural Affairs/Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China;College of Animal Science and Technology,Hebei Agricultural University,Baoding 071001,China
  • 2. State Key Laboratory of Animal Biotech Breeding/Key Laboratory of Animal(Poultry)Genetics Breeding and Reproduction of Ministry of Agriculture and Rural Affairs/Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China
  • 3. Joint International Research Laboratory of Agriculture and Agri-Product Safety,Ministry of Education/Institutes of Agricultural Science and Technology Development,Yangzhou University,Yangzhou 225009,China
  • 折叠

Abstract

Crossed beak is a complex mode of inheritance with prevalence ranging from 0.2 to 7.4％in at least 12 chicken strains worldwide.To reveal the intrinsic factors causing crossed beaks,genes expression patterns in bilateral mandibular condyle between affected and normal birds were characterized by RNA sequencing analysis in the present studies.Crossed beak was induced by short length of unilateral mandibular ramus,and a total of 110 differentially expressed genes were up-or down-regulated in the affected(short)mandibular condyle side as compared to the normal side.Carbonic anhydrase 2(CA2)and Carbonic anhydrase 13(CA13)were enriched in the carbonate dehydratase activity,and high-expressed in mandibular condyle and osteoblasts(P＜0.05).However,both were low-expressed in short mandibular condyle side of affected birds(P＜0.05).The carbonate dehydratase inhibitor experiments confirmed that there is positive association between the calcification and carbonic anhydrase isoenzymes.Quantitative analysis with cetylpyridinium chloride showed a decrease in calcification when the cells were transfected with an anti-CA13 shRNA.Our research suggested that CA2 and CA13 are down-calcified in short-side mandibular condyle,and caused mandibular ramus to grow slowly.CA2 and CA13 have the critical role in crossed beaks by regulating calcification of mandibular condyle.

Key words

chicken/crossed beak/carbonic anhydrase/calcification/CA2/CA13

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基金项目

Beijing Featured Livestock and Poultry Genetic Resources Preservation Project,China(202203310002)

China Agriculture Research System of MOF and MARA(CARS40)

Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(ASTIPIAS04)

Central Guidance on Local Science and Technology Development Fund of Hebei Province,China(236Z6602G)

出版年

2024
农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
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