农业科学学报(英文)2024，Vol.23Issue(7) ：2407-2420.DOI:10.1016/j.jia.2023.10.029

Genetic and pathogenic characterization of new infectious bronchitis virus strains in the GVI-1 and GI-19 lineages isolated in central China

Yuhan Yang Dou Wang Yaning Bai Wenyan Huang Shimin Gao Xingchen Wu Ying Wang Jianle Ren Jinxin He Lin Jin Mingming Hu Zhiwei Wang Zhongbing Wang Haili Ma Junping Li Libin Liang
农业科学学报(英文)2024，Vol.23Issue(7) ：2407-2420.DOI:10.1016/j.jia.2023.10.029
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  • NSTL
  • 万方数据

Genetic and pathogenic characterization of new infectious bronchitis virus strains in the GVI-1 and GI-19 lineages isolated in central China

Yuhan Yang 1Dou Wang 1Yaning Bai 1Wenyan Huang 1Shimin Gao 1Xingchen Wu 1Ying Wang 1Jianle Ren 1Jinxin He 1Lin Jin 1Mingming Hu 2Zhiwei Wang 2Zhongbing Wang 2Haili Ma 1Junping Li 1Libin Liang1
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作者信息

  • 1. College of Veterinary Medicine,Shanxi Agricultural University,Jinzhong 030801,China
  • 2. Shanxi Provincial Animal Disease Prevention and Control Center,Taiyuan 030000,China
  • 折叠

Abstract

Avian infectious bronchitis(IB)is a highly contagious infectious disease caused by infectious bronchitis virus(IBV),which is prevalent in many countries worldwide and causes serious harm to the poultry industry.At present,many commercial IBV vaccines have been used for the prevention and control of IB;however,IB outbreaks occur frequently.In this study,two new strains of IBV,SX/2106 and SX/2204,were isolated from two flocks which were immunized with IBV H120 vaccine in central China.Phylogenetic and recombination analysis indicated that SX/2106,which was clustered into the GI-19 lineage,may be derived from recombination events of the GI-19 and GI-7 strains and the LDT3-A vaccine.Genetic analysis showed that SX/2204 belongs to the GVI-1 lineage,which may have originated from the recombination of the GI-13 and GVI-1 strains and the H120 vaccine.The virus cross-neutralization test showed that the antigenicity of SX/2106 and SX/2204 was different from H120.Animal experiments found that both SX/2106 and SX/2204 could replicate effectively in the lungs and kidneys of chickens and cause disease and death,and H120 immunization could not provide effective protection against the two IBV isolates.It is noteworthy that the pathogenicity of SX/2204 has significantly increased compared to the GVI-1 strains isolated previously,with a mortality rate up to 60％.Considering the continuous mutation and recombination of the IBV genome to produce new variant strains,it is important to continuously monitor epidemic strains and develop new vaccines for the prevention and control of IBV epidemics.

Key words

infectious bronchitis virus/GI-19 lineage/GVI-1 lineage/complete genome/recombination/pathogenicity

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基金项目

National Natural Science Foundation of China(32202788)

Special Research Fund of Shanxi Agricultural University for Highlevel Talents,China(2021XG004)

Fund for Shanxi"1331 Project",China(20211331-13)

Shanxi Province Excellent Doctoral Work Award-Scientific Research Project,China(SXBYKY2021063)

Shanxi Province Excellent Doctoral Work Award-Scientific Research Project,China(SXBYKY2021005)

Shanxi Province Excellent Doctoral Work Award-Scientific Research Project,China(SXBYKY2022014)

earmarked fund for Modern Agroindustry Technology Research System of Shanxi Province,China(2023CYJSTX15-13)

Fundamental Research Program of Shanxi Province,China(202103021224156)

出版年

2024
农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
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