Genetic and pathogenic characterization of new infectious bronchitis virus strains in the GVI-1 and GI-19 lineages isolated in central China

扫码查看

原文链接

国家科技期刊平台
NETL
NSTL
万方数据

外文摘要：Avian infectious bronchitis(IB)is a highly contagious infectious disease caused by infectious bronchitis virus(IBV),which is prevalent in many countries worldwide and causes serious harm to the poultry industry.At present,many commercial IBV vaccines have been used for the prevention and control of IB;however,IB outbreaks occur frequently.In this study,two new strains of IBV,SX/2106 and SX/2204,were isolated from two flocks which were immunized with IBV H120 vaccine in central China.Phylogenetic and recombination analysis indicated that SX/2106,which was clustered into the GI-19 lineage,may be derived from recombination events of the GI-19 and GI-7 strains and the LDT3-A vaccine.Genetic analysis showed that SX/2204 belongs to the GVI-1 lineage,which may have originated from the recombination of the GI-13 and GVI-1 strains and the H120 vaccine.The virus cross-neutralization test showed that the antigenicity of SX/2106 and SX/2204 was different from H120.Animal experiments found that both SX/2106 and SX/2204 could replicate effectively in the lungs and kidneys of chickens and cause disease and death,and H120 immunization could not provide effective protection against the two IBV isolates.It is noteworthy that the pathogenicity of SX/2204 has significantly increased compared to the GVI-1 strains isolated previously,with a mortality rate up to 60％.Considering the continuous mutation and recombination of the IBV genome to produce new variant strains,it is important to continuously monitor epidemic strains and develop new vaccines for the prevention and control of IBV epidemics.

外文关键词：

infectious bronchitis virusGI-19 lineageGVI-1 lineagecomplete genomerecombinationpathogenicity

作者：

Yuhan Yang、Dou Wang、Yaning Bai、Wenyan Huang、Shimin Gao、Xingchen Wu、Ying Wang、Jianle Ren、Jinxin He、Lin Jin、Mingming Hu、Zhiwei Wang、Zhongbing Wang、Haili Ma、Junping Li、Libin Liang

展开 >

作者单位：

College of Veterinary Medicine,Shanxi Agricultural University,Jinzhong 030801,China

Shanxi Provincial Animal Disease Prevention and Control Center,Taiyuan 030000,China

基金：

National Natural Science Foundation of ChinaSpecial Research Fund of Shanxi Agricultural University for Highlevel Talents,ChinaFund for Shanxi"1331 Project",ChinaShanxi Province Excellent Doctoral Work Award-Scientific Research Project,ChinaShanxi Province Excellent Doctoral Work Award-Scientific Research Project,ChinaShanxi Province Excellent Doctoral Work Award-Scientific Research Project,Chinaearmarked fund for Modern Agroindustry Technology Research System of Shanxi Province,ChinaFundamental Research Program of Shanxi Province,China

项目编号：

322027882021XG00420211331-13SXBYKY2021063SXBYKY2021005SXBYKY20220142023CYJSTX15-13202103021224156

出版年：

2024

DOI：

10.1016/j.jia.2023.10.029

农业科学学报(英文)

中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD

影响因子：0.576

ISSN：2095-3119

年,卷(期)：2024.23(7)