Abstract
Pre-harvest sprouting(PHS)poses a significant global challenge to cereal production,impacting both yield and quality.In this study,we employed genome-wide association studies(GWAS)on diverse rice accessions to identify novel PHS-associated haplotypes.An assessment of 127 cultivated accessions for panicle germination(PHS)and detached grain germination(germination rate of detached grains at the 14th day(D14))revealed considerable phenotypic variation among rice ecotypes.GWAS analysis identified 91 significant signals at-log10(P-value)＞5,including 15 SNPs for PHS and 76 SNPs for D14.A subsequent linkage disequilibrium(LD)block-based GWAS analysis detected 227 significant SNPs for both traits,consisting of 18 nonsynonymous substitutions located on the coding regions of nine genes.Further haplotype analysis identified 32 haplotypes,with 10 specific to cultivated accessions,19 specific to the wild type,and three shared between them.A phenotypic assessment of major haplotypes revealed significant differences between resistant(Hap1 and Hap2)and susceptible haplotypes(Hap5,Hap27,and Hap28),distinguished by a G/A SNP within a novel gene,Os04g0545200.The identified haplotypes offer promising prospects for haplotype-based breeding aimed at enhancing PHS resistance in rice.
基金项目
National Research Foundation of Korea(NRF)grants funded by the Ministry of Science and Information and Communication Technol(NRF-2022R1A4A1030348)
National Research Foundation of Korea(NRF)grants funded by the Ministry of Science and Information and Communication Technol(2023R1A2C1004432)
Korea Institute of Planning and Evaluation for Technology in Food,Agriculture and Forestry(IPET)through the Digital Breeding(322060031HD020)
Cooperative Research Program for Agriculture Science and Technology Development,Rural Development Administration,Republic of(RS-2023-00222739)
国家科技期刊平台
NSTL
万方数据