农业科学学报(英文)2024，Vol.43Issue(8) ：2571-2588.DOI:10.1016/j.jia.2024.04.022

Fine-mapping and primary analysis of candidate genes associated with seed coat color in mung bean(Vigna radiata L.)

Qian Wang Huimin Cao Jingcheng Wang Zirong Gu Qiuyun Lin Zeyan Zhang Xueying Zhao Wei Gao Huijun Zhu Hubin Yan Jianjun Yan Qingting Hao Yaowen Zhang
农业科学学报(英文)2024，Vol.43Issue(8) ：2571-2588.DOI:10.1016/j.jia.2024.04.022
✕
  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据

Fine-mapping and primary analysis of candidate genes associated with seed coat color in mung bean(Vigna radiata L.)

Qian Wang 1Huimin Cao 1Jingcheng Wang 1Zirong Gu 1Qiuyun Lin 2Zeyan Zhang 1Xueying Zhao 1Wei Gao 1Huijun Zhu 1Hubin Yan 1Jianjun Yan 1Qingting Hao 1Yaowen Zhang1
扫码查看

作者信息

  • 1. College of Agronomy,Shanxi Agricultural University,Taiyuan 030031,China
  • 2. Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China
  • 折叠

Abstract

Seed coat color affects the appearance and commodity quality of mung beans(Vigna radiata L.).The substances that affect mung bean seed coat color are mainly flavonoids,which have important medicinal value.Mapping the seed coat color gene in mung beans would facilitate the development of new varieties and improve their value.In this study,an F2 mapping population consisting of 546 plants was constructed using Jilv9(black seed coat)and BIS9805(green seed coat).Using bulk segregated analysis(BSA)sequencing and kompetitive allele-specific PCR(KASP)markers,the candidate region related to seed coat color was finally narrowed to 0.66 Mb on chromosome(Chr.)4 and included eight candidate genes.Combined transcriptome and metabolome analyses showed that three of the eight candidate genes(LOC106758748,LOC106758747,and LOC106759075)were differentially expressed,which may have caused the differences in flavonoid metabolite content between Jilv9 and BIS9805.These findings can provide a research basis for cloning the genes related to seed coat color and accelerate molecular marker-assisted selection breeding in mung beans.

Key words

mung bean/seed coat color/bulk segregated analysis sequencing/transcriptome/metabolism/flavonoids

引用本文复制引用

基金项目

National Natural Science Foundation of China(32301928)

Basic Research Program of Shanxi Province,China(20210302124504)

China Agriculture Research System of MOF and MARA-Food Legumes(CARS-08-G10)

National Laboratory Project of Coarse Grain Germplasm Resources Innovation and Molecular Breeding,China(K462202040-01)

Ph D of Shanxi Agricultural University Scientific Research Startup Project,China(2021BQ43)

Scientific Research Project of Shanxi Agricultural University,China(YZGC098)

出版年

2024
农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
段落导航相关论文