首页|Fine-mapping and primary analysis of candidate genes associated with seed coat color in mung bean(Vigna radiata L.)

Fine-mapping and primary analysis of candidate genes associated with seed coat color in mung bean(Vigna radiata L.)

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
Seed coat color affects the appearance and commodity quality of mung beans(Vigna radiata L.).The substances that affect mung bean seed coat color are mainly flavonoids,which have important medicinal value.Mapping the seed coat color gene in mung beans would facilitate the development of new varieties and improve their value.In this study,an F2 mapping population consisting of 546 plants was constructed using Jilv9(black seed coat)and BIS9805(green seed coat).Using bulk segregated analysis(BSA)sequencing and kompetitive allele-specific PCR(KASP)markers,the candidate region related to seed coat color was finally narrowed to 0.66 Mb on chromosome(Chr.)4 and included eight candidate genes.Combined transcriptome and metabolome analyses showed that three of the eight candidate genes(LOC106758748,LOC106758747,and LOC106759075)were differentially expressed,which may have caused the differences in flavonoid metabolite content between Jilv9 and BIS9805.These findings can provide a research basis for cloning the genes related to seed coat color and accelerate molecular marker-assisted selection breeding in mung beans.

mung beanseed coat colorbulk segregated analysis sequencingtranscriptomemetabolismflavonoids

Qian Wang、Huimin Cao、Jingcheng Wang、Zirong Gu、Qiuyun Lin、Zeyan Zhang、Xueying Zhao、Wei Gao、Huijun Zhu、Hubin Yan、Jianjun Yan、Qingting Hao、Yaowen Zhang

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College of Agronomy,Shanxi Agricultural University,Taiyuan 030031,China

Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Haikou 571101,China

National Natural Science Foundation of ChinaBasic Research Program of Shanxi Province,ChinaChina Agriculture Research System of MOF and MARA-Food LegumesNational Laboratory Project of Coarse Grain Germplasm Resources Innovation and Molecular Breeding,ChinaPh D of Shanxi Agricultural University Scientific Research Startup Project,ChinaScientific Research Project of Shanxi Agricultural University,China

3230192820210302124504CARS-08-G10K462202040-012021BQ43YZGC098

2024

10.1016/j.jia.2024.04.022

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
年,卷(期)：2024.43(8)