农业科学学报(英文)2024，Vol.43Issue(8) ：2589-2604.DOI:10.1016/j.jia.2023.11.012

Deletion of a 1,049 bp sequence from the 5'UTR upstream of the SiHEC3 gene induces a seed non-shattering mutation in sesame

Ming Ju Guiting Li Qiuzhen Tian Hengchun Cao Qin Ma Yinghui Duan Hui Guo Zhanyou Zhang Yingying Huang Huili Wang Haiyang Zhang Hongmei Miao
农业科学学报(英文)2024，Vol.43Issue(8) ：2589-2604.DOI:10.1016/j.jia.2023.11.012
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Deletion of a 1,049 bp sequence from the 5'UTR upstream of the SiHEC3 gene induces a seed non-shattering mutation in sesame

Ming Ju 1Guiting Li 1Qiuzhen Tian 1Hengchun Cao 1Qin Ma 1Yinghui Duan 1Hui Guo 1Zhanyou Zhang 1Yingying Huang 1Huili Wang 1Haiyang Zhang 1Hongmei Miao1
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作者信息

  • 1. The Shennong Laboratory,Zhengzhou 450002,China;Henan Sesame Research Center,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China;Key Laboratory of Specific Oilseed Crops Genomics of Henan Province,Henan Sesame Research Center,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China
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Abstract

Sesame is a labor intensive crop with limited mechanized harvesting mainly due to the seed shattering(SS)trait.In this study,we performed a genetic analysis of the seed-shattering resistance(SR)trait with a SR sesame mutant 12M07.Unlike the SS type,the parenchyma cells in the abscission zone of the 12M07 mutant are arranged loosely but adhere to the seed coat.Inheritance analysis of six generations derived from 12M07(SR)×Xiangcheng Dazibai(SS)showed that the SR trait is recessive and controlled by a single gene pair.Association mapping of the F2 population with 888,619 variants(single-nucleotide polymorphisms(SNPs)and insertion-deletion(InDels))and 31,884 structural variations(SVs)determined that only SV12002 in the 5'upstream region of gene Sindi0765000(named SiHEC3)in Chr.3 was significantly associated with the SR trait.SiHEC3 encodes the bHLH transcription factor.A 1,049 bp deletion occurred in the 5'UTR of Sihec3 in 12M07.SiHEC3 is mainly expressed in developing placental tissues,with the expression peaking in capsules at 45 days after pollination.A dual-luciferase reporter assay in tobacco confirmed that the promoter activity of Sihec3 was reduced because of the deletion of the 1,049 bp promoter sequence.Protein-protein interaction network analysis showed that HEC3 is co-expressed with nine key proteins,such as SHATTERPROOF1(SHP1)and SEEDSTICK(STK)which participate in the secondary wall biosynthesis of the abscission layer in plants.The findings of this study show the important function of Sihec3 corresponding with the SR trait and supply the genetic information for breeding new varieties that are amenable to mechanized harvesting in sesame and other crops.

Key words

sesame(Sesamum indicum L.)/association mapping/seed shattering/structure variation/bHLH transcription factor

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基金项目

earmarked funding for the China Agriculture Research System of MOF and MARA(CARS-14)

Key Project of Science and Technology of Henan Province,China(201300110600)

Henan Province Specific Professor Position Program,China(SPPP2022)

Zhongyuan Scientist Workshop Construction,China(ZSWC2019)

Zhongyuan Scientist Workshop Construction,China(214400510026)

Innovation Scientist and Technician Troop Construction Project of Henan Province,China(ISTTCPHP2016)

Shennong Laboratory First Class Program,China(SN01-2022-04)

Key Research and Development Project of Henan Province,China(221111520400)

Innovation Scientists and Technicians Troop Construction Project of Henan Academy of Agricultural Sciences,China(2023TD04)

Henan Province Science and Technology Research and Development Plan Joint Fund,China(232301420108)

出版年

2024
农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
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