A novel histone methyltransferase gene CgSDG40 positively regulates carotenoid biosynthesis during citrus fruit ripening

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国家科技期刊平台
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外文摘要：The flesh color of pummelo(Citrus maxima)fruits is highly diverse and largely depends on the level of carotenoids,which are beneficial to human health.It is vital to investigate the regulatory network of carotenoid biosynthesis to improve the carotenoid content in pummelo.However,the molecular mechanism underlying carotenoid accumulation in pummelo is not fully understood.In this study,we identified a novel histone methyltransferase gene,CgSDG40,involved in carotenoid regulation by analyzing the flesh transcriptome of typical white-fleshed pummelo,red-fleshed pummelo and extreme-colored F1 hybrids from a segregated pummelo population.Expression of CgSDG40 corresponded to flesh color change and was highly coexpressed with CgPSY1.Interestingly,CgSDG40 and CgPSY1 are located physically adjacent to each other on the chromosome in opposite directions,sharing a partially overlapping promoter region.Subcellular localization analysis indicated that CgSDG40 localizes to the nucleus.Overexpression of CgSDG40 significantly increased the total carotenoid content in citrus calli relative to that in wild type.In addition,expression of CgPSY1 was significantly activated in overexpression lines relative to wild type.Taken together,our findings reveal a novel histone methyltransferase regulator,CgSDG40,involved in the regulation of carotenoid biosynthesis in citrus and provide new strategies for molecular design breeding and genetic improvement of fruit color and nutritional quality.

外文关键词：

pummeloSET domain proteinepigenetic regulationPSY1lycopenefruit qualityfruit color

作者：

Jialing Fu、Qingjiang Wu、Xia Wang、Juan Sun、Li Liao、Li Li、Qiang Xu

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作者单位：

National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops,College of Horticulture & Forestry Science,Huazhong Agricultural University,Wuhan 430070,China

Hubei Hongshan Laboratory,Wuhan 430070,China

Institute of Horticultural Sciences,Jiangxi Academy of Agricultural Sciences,Nanchang 330200,China

Plant Breeding and Genetics Section,School of Integrative Plant Science,Cornell University,New York 14853,USA

Robert W.Holley Center for Agriculture and Health,USDA-Agricultural Research Service,Cornell University,New York 14853,USA

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基金：

Major Special Projects and Key R&D Projects in Yunnan Province,ChinaNational Natural Science Foundation of ChinaFoundation of Hubei Hongshan Laboratory,ChinaKey Project of Hubei Provincial Natural Science Foundation,China

项目编号：

202102AE090054319250342021hszd0162021CFA017

出版年：

2024

DOI：

10.1016/j.jia.2024.03.068

农业科学学报(英文)

中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD

影响因子：0.576

ISSN：2095-3119

年,卷(期)：2024.43(8)