农业科学学报(英文)2024，Vol.43Issue(8) ：2723-2734.DOI:10.1016/j.jia.2023.12.030

A dicistrovirus increases pupal mortality in Spodoptera frugiperda by suppressing protease activity and inhibiting larval diet consumption

Meixue Sun Tong Li Yingjie Liu Kenneth Wilson Xingyu Chen Robert I.Graham Xianming Yang Guangwei Ren Pengjun Xu
农业科学学报(英文)2024，Vol.43Issue(8) ：2723-2734.DOI:10.1016/j.jia.2023.12.030
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A dicistrovirus increases pupal mortality in Spodoptera frugiperda by suppressing protease activity and inhibiting larval diet consumption

Meixue Sun 1Tong Li 2Yingjie Liu 3Kenneth Wilson 4Xingyu Chen 1Robert I.Graham 5Xianming Yang 6Guangwei Ren 1Pengjun Xu1
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作者信息

  • 1. Key Laboratory of Tobacco Pest Monitoring & Integrated Management,Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao 266101,China
  • 2. Institute of Plant Protection,Henan Academy of Agricultural Sciences,Zhengzhou 450000,China
  • 3. Staff Development Institute of China National Tobacco Corporation,Zhengzhou 450000,China
  • 4. Lancaster Environment Centre,Lancaster University,Lancaster LA14YW,UK
  • 5. Department of Rural Land Use,Scotland's Rural College,Craibstone Campus,Aberdeen AB01AB,UK
  • 6. State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China
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Abstract

Understanding interactions between viruses and their hosts is conducive to enabling better application of viruses as biocontrol agents.Certain viruses carried by parasitic wasps enhance the parasitic efficiency of wasp-larvae by protecting them against the immune system of their Lepidopteran host.However,the relationship between prey pests and viruses found in predatory natural enemies remains unclear.Herein,we report the interaction between Arma chinensis virus-1(AcV-1),originally isolated from a predatory natural enemy,Arma chinensis(Hemiptera:Pentatomidae),and one of its prey species,Spodoptera frugiperda(Lepidoptera:Noctuidae).The results showed that the AcV-1 virus appeared harmful to the novel host S.frugiperda by inhibiting larval diet consumption and increasing pupal mortality.Meanwhile,sequencing data indicated that the virus altered the gene expression profiles of S.frugiperda.KEGG analysis showed that the proteasome and phagosome pathways related to protein degradation and immune response were significantly enriched.Although the expression levels of digestive enzyme genes did not change significantly,the total protease activity of AcV-1 virus-positive individuals was significantly decreased,suggesting that the virus inhibited diet consumption of S.frugiperda via the down-regulation of digestive enzyme activities.These results indicate that a virus initially isolated in a predatory natural enemy can decrease the fitness of its prey species.The virus was found to impact the host proteasome and phagosome pathways related to protein degradation and immunity,providing a potential mechanism to enhance controlling efficiency.

Key words

Arma chinensis virus-1/diet consumption/fitness/transcriptome/protease activity

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基金项目

Major Special Projects for Green Pest Control,China(110202101028LS-03)

Major Special Projects for Green Pest Control,China(201938)

Major Special Projects for Green Pest Control,China(110202201017LS-01)

Major Special Projects for Green Pest Control,China(110202001035LS-04)

National Natural Science Foundation of China(31901893)

Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(ASTIP-TRIC04)

出版年

2024
农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
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