首页|Gga-miRNA-181-5p family facilitates chicken myogenesis via targeting TGFBR1 to block TGF-β signaling

Gga-miRNA-181-5p family facilitates chicken myogenesis via targeting TGFBR1 to block TGF-β signaling

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
MicroRNAs(miRNAs)have been demonstrated to control chicken skeletal muscle growth,however,the potential function of the miR-181-5p family in chicken myogenesis remains largely unknown.Here,our study identified the two chicken(Gallus gallus;Gga)miR-181-5p family members widely expressed in various tissues,specifically miR-181a-5p and miR-181b-5p.Besides,the breast muscles of fast-growing broilers expressed higher levels of miR-181a-5p and miR-181b-5p than those of slow-growing layers.Functionally,miR-181a-5p and miR-181b-5p both promote the expression level of myogenic factors including myogenin(MyoG),myogenic differentiation 1(MyoD1),and myosin heavy chain(MyHC),meanwhile accelerating the myotube formation of skeletal muscle satellite cells(SMSCs).Mechanistically,miR-181a-5p and miR-181b-5p directly bind to the 3'untranslated region(UTR)of the transforming growth factor beta receptor 1(TGFBR1)mRNA,further reducing the expression of TGFBR1.TGFBR1 is a key Transforming growth factor beta(TGF-β)signaling transduction receptor and had a negative function in muscle cell differentiation.Furthermore,knockdown of TGFBR1 facilitated the expression of chicken myogenic factors,boosted myotube formation,and decreased the SMAD family member 2/3(SMAD2/3)phosphorylation in chicken SMSCs.SMAD2/3 are downstream of TGF-β signaling,and miR-181a-5p and miR-181b-5p could reduce the expression of TGFBR1 to further diminish the SMAD2/3 phosphorylation.Our findings revealed that the miR-181-5p family targets TGFBR1 to break the TGF-β signaling transduction,which resulted in promoting chicken skeletal muscle development.

miRNA-181-5p familySMSCsdifferentiationTGFBR1TGF-β signaling

Xiaoxu Shen、Yongtong Tian、Wentao He、Can He、Shunshun Han、Yao Han、Lu Xia、Bo Tan、Menggen Ma、Houyang Kang、Jie Yu、Qing Zhu、Huadong Yin

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Key Laboratory of Livestock and Poultry Multi-omics,Ministry of Agriculture and Rural Affairs,College of Animal Science and Technology,Sichuan Agricultural University,Chengdu 611130,China

Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province,Sichuan Agricultural University,Chengdu 611130,China

College of Forestry,Sichuan Agricultural University,Chengdu 611130,China

College of Resources,Sichuan Agricultural University,Chengdu 611130,China

Triticeae Research Institute,Sichuan Agricultural University,Chengdu 611130,China

Key Laboratory for Animal Disease Resistance Nutrition of China,Institute of Animal Nutrition,Ministry of Education/Sichuan Agricultural University,Chengdu 611130,China

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National Key Research and Development Program of ChinaSichuan Science and Technology Program,ChinaSichuan Science and Technology Program,China

2022YFF100020202021YFYZ00072021YFYZ0031

2024

10.1016/j.jia.2023.05.025

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
年,卷(期)：2024.43(8)