农业科学学报(英文)2024，Vol.43Issue(8) ：2778-2791.DOI:10.1016/j.jia.2024.03.055

Genetic and biological properties of H9N2 avian influenza viruses isolated in central China from 2020 to 2022

Libin Liang Yaning Bai Wenyan Huang Pengfei Ren Xing Li Dou Wang Yuhan Yang Zhen Gao Jiao Tang Xingchen Wu Shimin Gao Yanna Guo Mingming Hu Zhiwei Wang Zhongbing Wang Haili Ma Junping Li
农业科学学报(英文)2024，Vol.43Issue(8) ：2778-2791.DOI:10.1016/j.jia.2024.03.055
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  • NSTL
  • 万方数据

Genetic and biological properties of H9N2 avian influenza viruses isolated in central China from 2020 to 2022

Libin Liang 1Yaning Bai 1Wenyan Huang 1Pengfei Ren 1Xing Li 1Dou Wang 1Yuhan Yang 1Zhen Gao 1Jiao Tang 1Xingchen Wu 1Shimin Gao 1Yanna Guo 1Mingming Hu 2Zhiwei Wang 2Zhongbing Wang 2Haili Ma 1Junping Li1
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作者信息

  • 1. College of Veterinary Medicine,Shanxi Agricultural University,Jinzhong 030801,China
  • 2. Shanxi Provincial Animal Disease Prevention and Control Center,Taiyuan 030000,China
  • 折叠

Abstract

The H9N2 subtype of avian influenza virus(AIV)is widely prevalent in poultry and wild birds globally,and has become the predominant subtype circulating in poultry in China.The H9N2 AIV can directly or indirectly(by serving as a"donor virus")infect humans,posing a significant threat to public health.Currently,there is a lack of in-depth research on the prevalence of H9N2 viruses in Shanxi Province,central China.In this study,we isolated 14 H9N2 AIVs from October 2020 to April 2022 in Shanxi Province,and genetic analysis revealed that these viruses belonged to 7 different genotypes.Our study on animals revealed that the H9N2 strains we identified displayed high transmission efficiency among chicken populations,and exhibited diverse replication abilities within these birds.These viruses could replicate efficiently in the lungs of mice,with one strain also demonstrating the capacity to reproduce in organs like the brain and kidneys.At the cellular level,the replication ability of different H9N2 strains was evaluated using plaque formation assays and multi-step growth curve assays,revealing significant differences in the replication and proliferation efficiency of the various H9N2 viruses at the cellular level.The antigenicity analysis suggested that these isolates could be classified into 2 separate antigenic clusters.Our research provides crucial data to help understand the prevalence and biological characteristics of H9N2 AIVs in central China.It also highlights the necessity of enhancing the surveillance of H9N2 AIVs.

Key words

avian influenza virus/H9N2/central China/pathogenicity/antigenicity

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基金项目

Fundamental Research Program of Shanxi Province,China(202103021224156)

National Natural Science Foundation of China(32202788)

Special Research Fund of Shanxi Agricultural University for Highlevel Talents,China(2021XG004)

special fund for Science and Technology Innovation Teams of Shanxi Province,China(202304051001041)

Science and Technology Innovation Program of Shanxi Agricultural University,China(2021BQ78)

Shanxi Province Excellent Doctoral Work Award-Scientific Research Project,China(SXBYKY2021005)

Shanxi Province Excellent Doctoral Work Award-Scientific Research Project,China(SXBYKY2021063)

Shanxi Province Excellent Doctoral Work Award-Scientific Research Project,China(SXBYKY2022014)

Fund for Shanxi"1331 Project",China(20211331-13)

earmarked fund for Modern Agroindustry Technology Research System of Shanxi Province,China()

出版年

2024
农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
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