Natural variation in the cytochrome c oxidase subunit 5B OsCOX5B regulates seed vigor by altering energy production in rice

Chengwei Huang ¹Zhijuan Ji ²Qianqian Huang ¹Liling Peng ¹Wenwen Li ¹Dandan Wang ¹Zepeng Wu ¹Jia Zhao ¹Yongqi He ¹Zhoufei Wang¹

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作者信息

1. Laboratory of Seed Science and Technology/Guangdong Key Laboratory of Plant Molecular Breeding/State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/South China Agricultural University,Guangzhou 510642,China
2. National Nanfan Research Institute(Sanya),Chinese Academy of Agricultural Sciences,Sanya 572025;China National Rice Research Institute,Hangzhou 310006,China
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Abstract

Seed vigor is a crucial trait for the direct seeding of rice.Here we examined the genetic regulation of seed vigor traits in rice,including germination index(GI)and germination potential(GP),using a genome-wide association study approach.One major quantitative trait locus,qGI6/qGP6,was identified simultaneously for both GI and GP.The candidate gene encoding the cytochrome c oxidase subunit 5B(OsCOX5B)was validated for qGI6/qGP6.The disruption of OsCOX5B caused the vigor traits to be significantly lower in Oscox5b mutants than in the japonica Nipponbare wild type(WT).Gene co-expression analysis revealed that OsCOX5B influences seed vigor mainly by modulating the tricarboxylic acid cycle process.The glucose levels were significantly higher while the pyruvic acid and adenosine triphosphate levels were significantly lower in Oscox5b mutants than in WT during seed germination.The elite haplotype of OsCOX5B facilitates seed vigor by increasing its expression during seed germination.Thus,we propose that OsCOX5B is a potential target for the breeding of rice varieties with enhanced seed vigor for direct seeding.

Key words

cytochrome c oxidase/natural variation/rice/seed vigor

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基金项目

USDA Dale Bumpers National Rice Research Center,Stuttgart,Arkansas,for providing rice seeds()

Hainan Province Science and Technology Special Fund,China(ZDYF2023XDNY086)

Project of Sanya Yazhou Bay Science and Technology City,China(SCKJ-JYRC-2022-87)

Natural Science Foundation of Guangdong Province,China(2023A1515012052)

Natural Science Foundation of Guangdong Province,China(2023A1515012092)

Guangzhou Science and Technology Plan Project,China(2023A04J1452)

Guangzhou Science and Technology Plan Project,China(2023A04J0749)

Double Firstclass Discipline Promotion Project,China(2021B10564001)

出版年

2024

农业科学学报(英文)

中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD

影响因子：0.576

ISSN：2095-3119

参考文献量1

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