首页|Natural variation in the cytochrome c oxidase subunit 5B OsCOX5B regulates seed vigor by altering energy production in rice

Natural variation in the cytochrome c oxidase subunit 5B OsCOX5B regulates seed vigor by altering energy production in rice

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  • 国家科技期刊平台
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  • NSTL
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Seed vigor is a crucial trait for the direct seeding of rice.Here we examined the genetic regulation of seed vigor traits in rice,including germination index(GI)and germination potential(GP),using a genome-wide association study approach.One major quantitative trait locus,qGI6/qGP6,was identified simultaneously for both GI and GP.The candidate gene encoding the cytochrome c oxidase subunit 5B(OsCOX5B)was validated for qGI6/qGP6.The disruption of OsCOX5B caused the vigor traits to be significantly lower in Oscox5b mutants than in the japonica Nipponbare wild type(WT).Gene co-expression analysis revealed that OsCOX5B influences seed vigor mainly by modulating the tricarboxylic acid cycle process.The glucose levels were significantly higher while the pyruvic acid and adenosine triphosphate levels were significantly lower in Oscox5b mutants than in WT during seed germination.The elite haplotype of OsCOX5B facilitates seed vigor by increasing its expression during seed germination.Thus,we propose that OsCOX5B is a potential target for the breeding of rice varieties with enhanced seed vigor for direct seeding.

cytochrome c oxidasenatural variationriceseed vigor

Chengwei Huang、Zhijuan Ji、Qianqian Huang、Liling Peng、Wenwen Li、Dandan Wang、Zepeng Wu、Jia Zhao、Yongqi He、Zhoufei Wang

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Laboratory of Seed Science and Technology/Guangdong Key Laboratory of Plant Molecular Breeding/State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources/South China Agricultural University,Guangzhou 510642,China

National Nanfan Research Institute(Sanya),Chinese Academy of Agricultural Sciences,Sanya 572025

China National Rice Research Institute,Hangzhou 310006,China

USDA Dale Bumpers National Rice Research Center,Stuttgart,Arkansas,for providing rice seedsHainan Province Science and Technology Special Fund,ChinaProject of Sanya Yazhou Bay Science and Technology City,ChinaNatural Science Foundation of Guangdong Province,ChinaNatural Science Foundation of Guangdong Province,ChinaGuangzhou Science and Technology Plan Project,ChinaGuangzhou Science and Technology Plan Project,ChinaDouble Firstclass Discipline Promotion Project,China

ZDYF2023XDNY086SCKJ-JYRC-2022-872023A15150120522023A15150120922023A04J14522023A04J07492021B10564001

2024

10.1016/j.jia.2023.06.018

农业科学学报(英文)
中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD
影响因子：0.576
ISSN：2095-3119
年,卷(期)：2024.23(9)