首页|Fine mapping and characterization of a major QTL for grain length,QGI.cau-2D.1,that has pleiotropic effects in synthetic allohexaploid wheat
Fine mapping and characterization of a major QTL for grain length,QGI.cau-2D.1,that has pleiotropic effects in synthetic allohexaploid wheat
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国家科技期刊平台
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Grain size is one of the determinants of grain yield,and identifying the genetic loci that control grain size will be helpful for increasing grain yield.In our previous study,a quantitative trait locus(QTL)for grain length(GL),QGI.cau-2D.1,was identified from an F2 population developed from the cross between the natural(TAA10)and synthetic(XX329)allohexaploid wheat.In the present study,we mainly fine mapped and validated its genetic effects.To this end,multiple near-isogenic lines(NILs)were obtained through marker-assisted selection with TAA10 as the recurrent parent.The secondary populations derived from 25 heterozygous recombinants were used for fine mapping of QGI.cau-2D.1,and the allele from XX329 significantly increased GL,thousand-grain weight(TGW),total spikelet number per spike(TSN)and spike compactness(SC).Using NILs for XX329(2D+)and TAA10(2D-),we determined the genetic and pleiotropic effects of QGI.cau-2D.1.The target sequences were aligned with the wheat reference genome RefSeq v2.1 and spanned an～0.9 Mb genomic region.TraesCS2D03G0114900(ortholog of Os03g0594700)was predicted as the candidate gene based on whole-genome re-sequencing and expression analyses.In summary,the map-based cloning of QGI.cau-2D.1 will be useful for improving grain weight with enhanced GL and TSN.
grain lengthtotal spikelet number per spikespike compactnessnear-isogenic linesQTLwheat
Frontiers Science Center for Molecular Design Breeding/Key Laboratory of Crop Heterosis and Utilization,Ministry of Education/Beijing Key Laboratory of Crop Genetic Improvement,China Agricultural University,Beijing 100193,China
National Plant Gene Research Centre,Beijing 100193,China
National Key Research and Development Program of China
国家科技期刊平台
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