A unique role of the pyrimidine de novo synthesis enzyme ODCase in Lysobacter enzymogenes

扫码查看

原文链接

国家科技期刊平台
NETL
NSTL
万方数据

外文摘要：Bacterial species of the genus Lysobacter are environmentally ubiquitous with strong antifungal biocontrol potential.Heat-stable antifungal factor(HSAF)secreted by the biocontrol bacterium Lysobacter enzymogenes OH11 has broad-spectrum and highly efficient antifungal activity.Studying the biosynthetic regulations of HSAF would lay an important foundation for strain engineering toward improved HSAF production.In this work,we demonstrate that Le0752,an orotidine-5'-phosphate decarboxylase enzyme(ODCase)catalyzing a pivotal step of the UMP de novo biosynthesis pathway,is vital for HSAF-mediated antimicrobial activities and growth of L.enzymogenes OH11,but not for twitching motility.This gene regulates the production of HSAF by affecting the expression of lafB,a key gene in the HSAF biosynthesis operon,through the transcription factor Clp.Interestingly,bioinformatics analysis revealed that Le0752 belongs to the Group Ⅲ ODCases,whereas its homologs in the closely related genera Xanthomonas and Stenotrophomonas belong to Group Ⅰ,which contains most ODCases from Gram-positive bacteria,Gram-negative bacteria and cyanobacteria.Moreover,the Group Ⅰ ODCase PXO_3614 from the Xanthomonas oryzae pv.oryzae PXO99Astrain complemented the Le0752 mutant in regulating HSAF-mediated antagonistic activity.Together,these results highlight the important requirement of de novo pyrimidine biosynthetic enzymes for antibiotic HSAF production in L.enzymogenes,which lays an important foundation for improving HSAF production via metabolic flow design and for dissecting the regulatory functions of bacterial ODCases.

外文关键词：

ODCasepyrimidine de novo synthesissecondary metabolite HSAFLysobacter enzymogenes

作者：

Mingming Yang、Yunxiao Tan、Jiabing Ma、Yingjia Zhao、Xia Yan、Nana Wang、Pingping Wang、Jiaqi Tan、Suilong Ai、Xiaofei Liang、Bangshuai Chang、Obadah E.A.Yousif、Chao Zhao、Bo Wang、Guoliang Qian、Lili Huang

展开 >

作者单位：

State Key Laboratory for Crop Stress Resistance and High-Efficiency Production,Northwest A&F University,Yangling 712100,China

College of Plant Protection,Northwest A&F University,Yangling 712100,China

College of Life Sciences,Northwest A&F University,Yangling 712100,China

College of Natural Resources and Environment,Northwest A&F University,Yangling 712100,China

Shaanxi Provincial Tobacco Corporation of China National Tobacco Corporation,Xi'an 710061,China

Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China

College of Plant Protection,Laboratory of Plant Immunity,Nanjing Agricultural University,Nanjing 210095,China

展开 >

基金：

National Natural Science Foundation of ChinaScience and Technology Major Project of China National Tobacco CorporationScience and Technology Project of Shaanxi Branch of China National Tobacco CorporationScience and Technology Project of Shaanxi Branch of China National Tobacco Corporation

项目编号：

32102283110202101056LS-16KJ-2021-02KJ-2022-04

出版年：

2024

DOI：

10.1016/j.jia.2023.11.047

农业科学学报(英文)

中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD

影响因子：0.576

ISSN：2095-3119

年,卷(期)：2024.23(9)