Knock-in of exogenous sequences based on CRISPR/Cas9 targeting autosomal genes and sex chromosomes in the diamondback moth,Plutella xylostella

Shanyu Li ¹Guifang Lin ¹Haoqi Wen ¹Haiyan Lu ¹Anyuan Yin ¹Chanqin Zheng ¹Feifei Li ¹Qingxuan Qiao ¹Lu Jiao ¹Ling Lin ¹Yi Yan ¹Xiujuan Xiang ¹Huang Liao ¹Huiting Feng ¹Yussuf Mohamed Salum ¹Minsheng You ¹Wei Chen ²Weiyi He¹

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作者信息

1. State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops/Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops/International Joint Research Laboratory of Ecological Pest Control,Ministry of Education,Institute of Applied Ecology,Fujian Agriculture and Forestry University,Fuzhou 350002,China
2. Ganzhou Key Laboratory of Greenhouse Vegetable,Gannan Normal University,Ganzhou 341000,China
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Abstract

Genetic pest control strategies based on precise sex separation and only releasing sterile males can be accomplished by site-specific genome editing.In the current study,we showed that the mutation of single-allele Pxfl(2)d can significantly impair the normal mating behavior and testis development in male adults of the notorious cruciferous insect pest Plutella xylostella,in addition to its known functions in the ovarian development in female adults and egg hatching.Subsequent CRISPR/Cas9-based knock-in experiments revealed that site-specific integration of an exogenous green fluorescent protein(GFP)gene into autosomal Pxfl(2)d for labelling mutants could be achieved.However,this gene is not a suitable target for GFP insertion to establish a genetically stable knock-in strain because of the severe decline in reproductive capacity.We further screened for the W-chromosome-linked and Z-chromosome-linked regions to test the knock-in efficiency mediated by CRISPR/Cas9.The results verified that both types of chromosomes can be targeted for the site-specific insertion of exogenous sequences.We ultimately obtained a homozygous knock-in strain with the integration of both Cas9 and cyan fluorescent protein(CFP)expression cassettes on a Z-linked region in P.xylostella,which can also be used for early sex detection.By injecting the sgRNA targeting Pxfl(2)d alone into the eggs laid by female adults of the Z-Cas9-CFP strain,the gene editing efficiency reached 29.73％,confirming the success of expressing a functional Cas9 gene.Taken together,we demonstrated the feasibility of the knock-in of an exogenous gene to different genomic regions in P.xylostella,while the establishment of a heritable strain required the positioning of appropriate sites.This study provides an important working basis and technical support for further developing genetic strategies for insect pest control.

Key words

cruciferous specialist/fl(2)d/CRISPR/Cas9/knock-in/sex chromosome

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基金项目

National Natural Science Foundation of China(32172503)

National Natural Science Foundation of China(32260721)

Natural Science Foundation of Fujian Province,China(2023J01069)

State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,China(SKL2022001)

Innovation Fund of Fujan Agriculture and Forestry University,China(KFB23014A)

Undergraduate Training Program for Innovation and Entrepreneurship of Fujian Province,China(S202210389101)

出版年

2024

农业科学学报(英文)

中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD

影响因子：0.576

ISSN：2095-3119

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