首页|Map-based cloning of qLPA01.1,a favorable allele from the Gossypium tomentosum chromosome segment line
Map-based cloning of qLPA01.1,a favorable allele from the Gossypium tomentosum chromosome segment line
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Map-based cloning of qLPA01.1,a favorable allele from the Gossypium tomentosum chromosome segment line
Cotton is an important natural fiber crop worldwide which plays a vital role in our daily life.High yield is a constant goal of cotton breeding,and lint percentage (LP) is one of the important components of cotton fiber yield.A stable QTL controlling LP,qLPA01.1,was identified on chromosome A01 from Gossypium hirsutum introgressed lines with G.tomentosum chromosome segments in a previous study.To fine-map qLPA01.1,an F2 population with 986 individuals was established by crossing G.hirsutum cultivar CCRI35 with the chromosome segment substitution line HT_390.A high-resolution genetic map including 47 loci and spanning 56.98 cM was constructed in the QTL region,and qLPA01.1 was ultimately mapped into an interval corresponding to an～80 kb genome region of chromosome A01 in the reference genome,which contained six annotated genes.Transcriptome data and sequence analysis revealed that S-acyltransferase protein 24 (GoPAT24) might be the target gene of qLPA01.1.This result provides the basis for cotton fiber yield improvement via marker-assisted selection (MAS) and further studies on the mechanism of cotton fiber development.
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