Long non-coding RNA FPFSC promotes immature porcine Sertoli cell growth through modulating the miR-326/EHMT2 axis

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外文摘要：Sertoli cells are indispensable for guaranteeing normal spermatogenesis and male fertility.Although a huge number of long non-coding RNAs(lncRNAs)are identified from developing porcine testicular tissues and have been predicted with crucial regulatory roles in spermatogenesis,their functions and regulatory mechanisms are still in infancy.Herein,we mainly explored the regulatory and functional roles of lncFPFSC in proliferation and apoptosis of immature porcine Sertoli cells.The results demonstrated that lncFPFSC was predominantly located in the cytoplasm of immature porcine Sertoli cells.lncFPFSC overexpression promoted cell cycle progression and cell proliferation,as well as inhibited cell apoptosis,whereas siRNA-induced lncFPFSC knockdown resulted in the opposite effects.Mechanistically,lncFPFSC acted as a sponge for miR-326.Overexpression of miR-326 inhibited cell proliferation and induced cell apoptosis,which further abolished the effects of lncFPFSC overexpression.The euchromatic histone-lysine N-methyltransferase 2(EHMT2)gene was directly targeted by miR-326,and its mRNA and protein expressions were both negatively regulated by miR-326 in immature porcine Sertoli cells.Then,siRNA-induced EHMT2 knockdown resulted a similar effect of miR-326 inhibition.Collectively,lncFPFSC promoted proliferation and inhibited apoptosis in immature porcine Sertoli cells through modulating the miR-326/EHMT2 axis.This study expanded our understanding of non-coding RNAs in participating porcine spermatogenesis through deciding the fate of Sertoli cells,and the competing endogenous RNA(ceRNA)network,and provided new molecular markers to treat Sertoli cell disorder inducing male infertility.

作者：

Dan Chu、Bin Chen、Bo Weng、Saina Yan、Yanfei Yin、Xiangwei Tang、Maoliang Ran

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作者单位：

Hunan Provincial Key Laboratory for Genetic Improvement of Domestic Animal,College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128,China

Xiangxi Vocational and Technical College for Nationalities,Xiangxi 416007,China

基金：

special funds for Changsha Municipal Natural Science Foundation,ChinaHunan Provincial Natural Science Foundation of ChinaHunan Provincial Natural Science Foundation of ChinaKey R&D Projects

项目编号：

kq22022292023JJ302962023JJ602472020NK2024

出版年：

2024

DOI：

10.1016/j.jia.2023.06.026

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[2]	Wang, Qiugen,Cao, Lei,Wang, Jiandong.MiR-326 is a diagnostic biomarker and regulates cell survival and apoptosis by targeting Bcl-2 in osteosarcoma[J].Biomedicine & pharmacotherapy,2016,848.
[3]	Liang, Xuan,Li, Zhaolun,Men, Qunli,et al.miR-326 functions as a tumor suppressor in human prostatic carcinoma by targeting Mucin1[J].Biomedicine & pharmacotherapy,2018,10810.
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同作者其他文献

农业科学学报(英文)

中国农业科学院农业信息研究所

农业科学学报(英文)

CSTPCD

影响因子：0.576

ISSN：2095-3119

年,卷(期)：2024.23(11)

Dan Chu,Bin Chen,Bo Weng,et al.Long non-coding RNA FPFSC promotes immature porcine Sertoli cell growth through modulating the miR-326/EHMT2 axis[J].农业科学学报(英文),2024,23(11):3830-3842.DOI:10.1016/j.jia.2023.06.026.