谷氨酸菌体蛋白的酶解条件优化
Optimization of the enzymatic hydrolysis of cell protein of Corynebacteria glutamic acid
周大伟 1肖冬光 1郭学武 1吕鸿雁 1杜丽平1
作者信息
- 1. 工业微生物教育部重点实验室,天津市工业微生物重点实验室,天津科技大学生物工程学院,天津,300457
- 折叠
摘要
采用响应面法对谷氨酸菌体蛋白的酶解条件进行优化,以蛋白水解度为参考指标,确定晟佳酶解工艺为酸性蛋白酶与β-葡聚糖酶的添加比例为3:1,总加酶量为2%(相对于底物),pH值为4.0,水解温度50℃,底物浓度15%,水解时间10h,在上述条件下,蛋白水解度达到18.01%.
Abstract
Using hydrolysis of protein as reference, enzymatic hydroly sis of cell protein of Corynebacteria glutamic acid was optimized with Plackett-Burman design.The results showed that optimum conditions were as follows: ratios of acidic protease and β-glucanase 3:1, total addition of enzymes 2％, temperature 50℃, pH value 4.0, concentration of substrate 15％ and hydrolysis 10h.Under these conditions, the hydrolysis degree of protein reached 18.01％.
关键词
谷氨酸菌体/酶解/响应面Key words
Corynebacteria glutamic acid/enzymatic hydrolysis/response surface引用本文复制引用
出版年
2011
国家科技期刊平台
NSTL
维普
万方数据