Optimum cultivation conditions for κ-carrageenanase produced by Cellulophaga lytica strain N5-2
Microbial enzyme production is a complex physiological and biochemical process, and different cultivation conditions directly affect the metabolism of bacteria, which thus affects enzyme production and activity. Optimization of cultivation conditions was a prerequisite to improve enzyme production. The medium formula and fermentation conditions were optimized by single factor experiments. The best medium formula was as follows: NaCl 2%, MgSO4·7H2O 0.05%, CaCl2 0.02%, KH2PO4 0.1%, FeSO4 0.002%, 0.2% K-carrageenan as carbon source, 0.3% peptone as nitrogen source. The optimal cultivation conditions were as follows: filling volume 50ml/250ml flask, inoculum size 4%, culture temperature 35t, 100r/min cultivated for 16h. Under these conditions, enzyme activity was 0.25U/ml, which was 8.3 times as that of the original conditions. It provided the experimental foundation and theoretical basis for large quantity of K-carrageenase and further study.
k-carrageenanaseoptimization of fermentation conditionsmarine Cellulophaga lytica
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