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酱油生产中应用米曲霉和黑曲霉混合制曲的探索

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该文考察了米曲霉和黑曲霉在单独制曲及混合制曲条件下,成曲的中性蛋白酶、酸性蛋白酶、糖化酶及孢子数,探讨了酱油多菌种制曲的可行性.实验以豆粕、麸皮为原料,以AS3.042米曲霉、AS3.350黑曲霉为菌种,探讨了2种制曲方式及不同配比制曲效果的影响.结果表明,米、黑曲霉分开制曲比混合接种制曲所得成曲效果好,米、黑曲霉分别以33℃制曲42h、48h得成品曲,再以2∶1混合所得成曲可获得较佳的酶活力,曲料总蛋白酶活力2748U/g与对照值相当,而酸性蛋白酶及糖化酶活力分别达712U/g、1090U/g,较对照分别提高55.8%、25.4%.
Application of a mixed koji-making system by Aspergillus oryzae and Aspergillus niger in soy sauce production
The neutral protease activity, acid protease activity, glucoamylase activity and the number of spores in separate koji-making and mixed ko-ji-making by Aspergillus oryzae and Aspergittus niger were measured in this paper. The feasibility of multi-strains for soy sauce koji was discussed. The effects of using AS3.042 and AS3.350 as fermented culture on koji-making were investigated. The results showed that separate koji-making was better than mixed koji-making in all respects. The optimal koji-making conditions were obtained as follows: fermentation temperature 33°C, fermentation time 42h for A. Oryzae and 48h for A. Niger, and the ratio of A. Oryzae to A. Nigerl'A. Under these conditions, total protease activity of koji was 2748U/g, which was similar to the value of the control. The acid protease and glucoamylase activity were 712U/g and 1090U/g, respectively, which was increase by 55.8% and 25.4% compared to that of koji-making by A. Oryzae, respectively.

two molds koji-makingseparated koji-makingmixed koji-making

李保英、姜佳丽、蒋予箭

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浙江工商大学食品与生物工程学院,浙江省食品安全重点实验室,浙江杭州310012

双菌种制曲 分开制曲 混合制曲

浙江省成果转化资金项目

2011D70044

2011

中国酿造
中国调味品协会 北京食品科学研究院

中国酿造

CSTPCD
影响因子:0.759
ISSN:0254-5071
年,卷(期):2011.(12)
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