Screening of high ascomycin-producing strains by N+ ion implantation and NaNO2 complex mutation
In order to obtain high ascomycin-producing strains, the strain NJWGH1322 was used as original strain for mutation by N+ ion implantation and NaNO2. The optimal amount of ion beam was 200× 104 ions/cm2 and the mutation time of NaNO2 was 5min. The both method were combined to do complex mutation. The results showed that the effect of complex mutation was much better than that of single mutation. A high ascomycin-producing strain was obtained using complex mutation. The production of ascomycin was increased by 43.1% compared to that of the original strain. The strain showed good stability after five generations.
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