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羊肚菌液体发酵培养基成分及培养条件的优化

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采用单因素和正交试验设计法对羊肚菌液体发酵培养基成分及培养条件的优化进行了研究.结果表明,羊肚菌液体发酵培养基最佳配方为可溶性淀粉2.5%,酵母膏2.5%,MgSO40.1%,KH2PO4 0.2%,VBt0.2%;最适培养条件:pH值为7,发酵温度28℃、转速140r/min,装液量100mL/250mL,摇床振荡培养7d,在最佳条件下所生产的菌丝生物量为3.438g/200mL.羊肚菌液体发酵培养基成分和发酵条件的优化工艺能高效生产菌丝体,为以后开发羊肚菌真菌资源产品奠定了基础.
Morchella liquid fermentation culture medium and optimization of cultivation conditions
In this paper,we researched studied on Morchella liquid fermentation culture medium and cultivation conditions of optimization by using the single factor and orthogonal experiment design method.The results showed that optimal Morchella liquid fermentation medium was starch 2.5%,yeast extract 2.5%,MgSO4 0.1%,KH2PO4 0.2% and VB1 0.2%; The optimum cultivation conditions were pH value 6,fermentation temperature 28℃,rotational speed 140r/min,fluid amount 100ml/250ml,and shaking culture 7d.Under the optimum conditions the production of mycelial biomass reached 3.438g/200ml.Morchella liquid fermentation medium and fermentation conditions for the optimization of the mycelium of production was efficient,and established the foundation for later development of Morchella fungi resources products.

Morchella esculentaliquid fermentationmycelium biomasssingle-factororthogonal experiments

杨建、张光宇、杜秀娟、杨玉红

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沈阳农业大学生物科学技术学院,辽宁沈阳110161

羊肚菌 液体发酵 菌丝生物量 单因素 正交试验

2013

中国酿造
中国调味品协会 北京食品科学研究院

中国酿造

CSTPCD
影响因子:0.759
ISSN:0254-5071
年,卷(期):2013.32(10)
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