首页|传统发酵豆酱中产α-淀粉酶菌株的筛选

传统发酵豆酱中产α-淀粉酶菌株的筛选

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α-淀粉酶广泛分布于动物、植物和微生物中,能水解淀粉产生糊精、麦芽糖、低聚糖和葡萄糖等,是食品工业中应用最为广泛的酶制剂之一.从东北三省传统的家庭自制豆酱上获得纯培养真菌389株,通过可溶性淀粉平板初筛获得具有α-淀粉酶活性的菌株34株,其中曲霉属的真菌14株、青霉12株、毛霉2株、镰孢菌2株、帚霉1株、犁头霉l株、木霉2株.米曲霉(Aspergillus oryzae)、橘灰青霉(Penicillium aurantiogriseum)和疣状青霉(Penicillium verrucosum)出现频率较高,是豆酱发酵过程中产生α-淀粉酶的优势种群.
Screening of α-amylase production strains from traditional fermented soybean paste
α-amylase is widely distributed in animals,plants and microbes; it can hydrolyze starch and generate dextrin,maltose,oligosaccharides,and glucose,it is one of the most widely-used enzymes in food industry.In the study,389 strains of pure culture fungi were obtained from the traditional homemade soybean paste in three northeast provinces; 34 strains with α-amylase activity were preliminarily screened through soluble starch plate method,including 14 strains of Aspergillus,12 strains of Penicillium,2 strains of Mucor,2 strains of Fusarium,1 strain of Scopulariopsis,1 strain of Absidia,and 2 strains of Trichoderma.The frequency of occurrence of A.oryzae,P.aurantiogriseum and P.verrucosum was higher and they were dominant strains producing α-amylase in the fermentation process.

soybean pasteα-amylase activityAspergillusPenicillium

孙晓东、吕国忠、栾雨时、陈嵘、赵志慧

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大连理工大学 生命科学与技术学院,辽宁 大连 116024

大连民族大学环境与资源学院,辽宁 大连116600

辽宁出入境检验检疫局,辽宁大连116600

豆酱 α-淀粉酶活性 曲霉菌 青霉菌

国家自然科学基金青年基金

31200014

2015

中国酿造
中国调味品协会 北京食品科学研究院

中国酿造

CSTPCD北大核心
影响因子:0.759
ISSN:0254-5071
年,卷(期):2015.34(5)
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