Ultrasound-assisted extraction technology of protein from fermented okara
Using okara as raw material, Aspergillus niger as starter to ferment okara, and then protein was extracted from fermented okara by ultrasound-assisted alkali solution acid precipitation method and single alkali solution acid precipitation method.The single factor and orthogonal experiment results showed that ultrasound-assisted alkali solution acid precipitation method was better than single alkali solution acid precipitation in increasing okara extraction rate and reducing the extraction time greatly.The optimal condition were as follows: ultrasound temperature 55 ℃, alkali extraction time 90 min, NaOH 0.3 mol/L, ultrasonic power 300 W, solid-liquid ratio 1:24 (g:ml).Under this condition, the optimal okara protein extraction rate was 83.84％.
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