Screening of β-glucosidase-producing non-Saccharomyces yeasts with and its enzymatic characteristics
Through the screening of yeasts in grapes from Xinjiang Shihezi wine-producing regions,the strains were isolated and purified by yeast peptone dextrose (YPD) medium,and isolated strains were identified by molecular biology method.Using p-Nitrophenyl-β-D-glucopyranoside (p-NPG) as substrate,the β-glucosidase-producing yeasts were screened,and one strain of non-Saccharomyces yeast with high glycosidase-production was obtained and identified as Pichia sp.The β-D-glucopyranoside producing characteristics of the strain were researched.The results showed that the optimum initial pH of β-glucosidase was 5.0,the optimum temperature of enzyme production was 30 ℃,reaction temperature was 15 min and the optimum content of glucose was 15 g/100 ml.
NETL
NSTL
万方数据
维普
