Using the nematocidal activity as evaluation index,on the basis of 5 L and 30 L fermentation research,the strain Sr18 (Syncephalastrum racemosum) was cultured in the scale of 200 L and 5 t fermenter.The results showed that the fermentation conditions of 200 L fermenter was as follows:inoculum 4％,fermentation temperature 26 ℃,vessel pressure 0.05-0.12 MPa,air flow rate 4-9 m3/h,rotational speed 120-198 r/min and fermentation cycle 48 h.The fermentation conditions in the scale of 5 t fermenter was as follows:inoculum 4％,fermentation temperature 26 ℃,tank pressure 0.03 MPa,air flow rate 65-80 m3/h,rotational speed 80-90 r/rmin and femmentation cycle 40 h.Under the fermentation conditions,the nematicidal activity of one time diluted fermentation broth could reach 100％,which indicated that the fermentation parameters were suitable and controllable.
NETL
NSTL
万方数据
维普
