Isolation, identification and phylogenetic analysis of yeast from traditional Xinjiang fermented yogurt
47 yeast strains were isolated from 18 samples of traditional home-made Xinjiang Kazakh naturally fermented yogurt,and then the strains were further identified by morphology analysis,physiological and biochemical identification,PCR-DGGE and 26S rDNA sequencing analysis,finally the phylogenetic tree was constructed.The results showed that the strains were identified as follows:18 strains of Pichia kudnavzevii,10 strains of Trichosporon asahii,7 strains of Saccharomyces cerevisiae,4 strains of Trichosporon coremiiforme,4 strains of Kluyveromyces marxianus,2 strains of Issatchenkia orientalis,1 strain of Pichia fermentans,1 strain of Candida parapsilosis.Therefore,the identification results were significant to recognize and utilize the resource of yeast of traditional Kazakh fermented yogurt.
traditional fermented yogurt26S rDNA D1/D2screeningisolation and identificationPCR-DGGE