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新疆传统发酵酸奶中酵母菌的分离鉴定及系统发育分析

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从新疆塔城地区哈萨克族不同牧场家庭中自然发酵牛乳采集18份样品,从中分离筛选得到47株酵母菌,并进行形态鉴定、生理生化鉴定、聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE),26S rDNA分子生物学鉴定,构建系统发育树.结果表明,47株菌中包括库德毕赤酵母(Pichia kudriavzevii) 18株,阿萨希丝孢酵母菌(Trichosporon asahii) 10株,酿酒酵母(Saccharomyces cerevisiae)7株,丝孢酵母(Tnchosporon coremiiforme)4株,马克斯克鲁维酵母(Kluyveromyces marxianus)4株,东方伊萨酵母(Issatchenkia orientalis)2株,发酵毕赤酵母(Pichia fermentans)1株,近平滑假丝酵母(Candida parapsilosis)1株.鉴定结果对进一步认识并利用哈萨克族传统发酵酸奶中酵母菌资源具有重要意义.
Isolation, identification and phylogenetic analysis of yeast from traditional Xinjiang fermented yogurt
47 yeast strains were isolated from 18 samples of traditional home-made Xinjiang Kazakh naturally fermented yogurt,and then the strains were further identified by morphology analysis,physiological and biochemical identification,PCR-DGGE and 26S rDNA sequencing analysis,finally the phylogenetic tree was constructed.The results showed that the strains were identified as follows:18 strains of Pichia kudnavzevii,10 strains of Trichosporon asahii,7 strains of Saccharomyces cerevisiae,4 strains of Trichosporon coremiiforme,4 strains of Kluyveromyces marxianus,2 strains of Issatchenkia orientalis,1 strain of Pichia fermentans,1 strain of Candida parapsilosis.Therefore,the identification results were significant to recognize and utilize the resource of yeast of traditional Kazakh fermented yogurt.

traditional fermented yogurt26S rDNA D1/D2screeningisolation and identificationPCR-DGGE

乔传丽、蒋彩虹、金丹、蒋艾廷、卢士玲、李王强、李宝坤

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石河子大学食品学院,新疆石河子832000

传统发酵酸奶 26S rDNA D1/D2 筛选 分离鉴定 聚合酶链式反应-变性梯度凝胶电泳

国家自然科学基金地区项目

31460007

2017

中国酿造
中国调味品协会 北京食品科学研究院

中国酿造

CSTPCD北大核心
影响因子:0.759
ISSN:0254-5071
年,卷(期):2017.36(4)
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