Microbial diversity of culturable microorganism from Yangshao pottery-flavor Baijiu Daqu
The diversity of culturable microorganism in Pottery-flavor Baijiu (Chinese liquor) Daqu was studied.The Daqu microorganisms in Potteryflavor Baijiu were isolated by tissue culture method.When microbial DNA was extracted,yeast universal primers NL1/NL4 was used for 26S rRNA sequences amplification,bacteria universal primer 799F/1492R was used for 16S rRNA sequences amplification,mold universal primer ITS1/ITS4 was used for 28S rRNA sequences amplification,the sequences were analyzed and results were submitted,to construct phylogenetic tree.Results showed that there were 213 yeasts,386 bacteria and 73 mould strains isolated from Pottery-flavor Baijiu Daqu,including 72 yeasts species,77 bacteria species,and 7 moulds species.Based on sequencing results,the isolated strains were classified into 21 operational taxonomic units (OUTs).The culturable microorganism in Yangshao Pottery-flavor Baijiu Daqu had microbial diversity,which provided theoretical foundation for fermentation and utilization.
Pottery-flavor BaijiuDaqumicrobial diversityphylogenetic tree
NETL
NSTL
万方数据
维普
