Screening, identification and metabolites analyses of Bacillus in pit mud of Luzhou-flavor Baijiu
Three strains of bacteria were screened from the pit mud of Luzhou-flavor Baijiu.Through phospholipid fatty acid (PLFA) identification,the strains were identified as Lysinibacillus sphaerieus,Brevibacillus brevis and Paenibacillus larvae subsup.pulvifaciens,respectively.The fermentation products of three strains were analyzed by solid phase microextraction combined with gas chromatography-mass spectrometry (SPME-GC-MS).The results showed that three strains had strong ability to produce flavor substances which had various kinds,mainly for aromatic compounds (including alcohols and ketones).As the trace components in Baijiu,the aromatic compounds played an putting incense,helping incense and blending incense role in the formation of Baijiu flavor.Therefore,the metabolic products of three strains had certain effect on the formation of flavor substances in Luzhou-flavor Baijiu.