Screening of high tolerance Saccharomyces cerevisiae and its tolerance
Adding excellent resistant strains can effectively improve the production efficiency of soy sauce and quality and flavor of products.Two strains of yeast resistant high temperature,hyperosmotic and high acid were screened and were used in the fermentation of soy sauce.The number and morphology of the colonies of different dilutions were compared by the dilution plate after the high temperature heat shock of the yeast,and the tolerance of each strain was compared by the growth on the hyperosmotic plate and the high acid plate and the growth curve determination in the resistance medium.The results of dilute point plate experiments and growth curve showed that the colony morphology and size of Saccharomyces cerevisiae L-19 and L-38 in the conditions of 55 ℃ heat shock and solid plate (containing 6％ NaC1,0.6％ acetic acid and 5％ lactic acid) were better than that of soy sauce yeast,and the growth rates of S.cerevisiae L-19 and L-38 in a liquid medium containing high salt and high acid were higher than that of soy sauce yeast.Therefore,two strains of yeast with high tolerance were successfully screened.
NETL
NSTL
万方数据
维普
