Screening and identification of multi-antibiotics-degrading lactic acid bacteria and study on its characteristics
To study and screen the hight effective antibiotics-degrading lactic acid bacteria,a lactic acid bacteria strain P3-4 was isolated from soil by HPLC and microbiology methods.According to the colonies,cells morphology,the results of the Biolog automated microbial analysis system and 16S rDNA gene sequence similarity analysis,the isolated strain P3-4 was identified as Pediococcus acidilactici.The strain P3-4 had the antibiotics degradation ability in MRS medium,and could degrade erythromycin,roxithromycin and spiramycin.The degradation rate of erythromycin,roxithromycin and spiramycin were 92.1%,68.9%,and 82.8%,respectively.These results had theoretical support for the further development and use of strain P3-4.